Software User Manual

Analyzing Melt Data

Precision Melt Data Tab

The Precision Melt Data tab shows spreadsheets that describe the melt data collected in each

well. Select one of the six options to show the data in different formats.

• Results. Displays a spreadsheet view of the data

•Charts. Displays multiple data charts in a single sheet

• Plate view. Displays a view of the data in each well as a plate map

• Raw RFU. Displays the RFU value in each well for each temperature

• Normalized RFU. Displays the normalized RFU value in each well for each temperature

• Difference RFU. Displays the difference RFU value in each well for each temperature

TIP: Right-click any spreadsheet for options. Sort the data in any spreadsheet by

right-clicking and choosing the Sort option. Click View/Edit Plate to open the

Plate Editor, and change the contents of any wells in the plate.

Results Spreadsheet

Select a Results spreadsheet (Figure 32) to see data for each well in the plate.

Figure 32. Precision Melt Data tab with Results spreadsheet selected.

The Results spreadsheet includes the information listed in Table 16.

Table 16. Results spreadsheet contents

Information Description

Well Well position in the plate

Content A combination of the Sample Type and Replicate # loaded in

the well in the Plate Editor

Sample Sample name loaded in the well in the Plate Editor

Cluster Name of the cluster assignment for the well

Percent Confidence Confidence percent value as an integrity check for auto-called

results

Call Type Automatic or Manual. Automatic means the software assigned

the cluster call for the well. Manual means the user assigned

the cluster call for the well