Accessories

Microseal
®
‘B’ Adhesive Seals
for PCR Plates
Microseal ‘B’ adhesive seals provide a tight sealing option for PCR in thin-wall PCR plates.
The aggressive adhesive allows secure sample storage or transport, before and after cycling.
The clear polyester backing allows easy inspection of sample wells and effective light
transmission for optical assays. Removal of the liner exposes a pristine sealing surface that
is certified free of RNase, DNase, and human DNA. Sealing during PCR requires pressure
applied by a heated lid. The robust adhesive is effective from –40°C to 110°C.
Microseal ‘B’ clear seals can be used with all polypropylene and polystyrene PCR plates.
This sealer is not recommended for Concord
™
-type polycarbonate PCR plates due to its
strong adhesion to the flat upper surface of those plates. The perforated end-tabs should
be removed when using this sealer on Microseal semi-skirted PCR plates (MSS-9601) or in
automated plate handlers.
Directions For Use
1. Remove gold-colored liner and place adhesive surface over wells.
2. Apply pressure with fingers or a soft rubber roller (#MSR-0001) to ensure
complete contact with each well.
3. Close heated lid and tighten firmly to ensure proper sealing. For MJ-line cyclers with
adjustable lids, turn thumbwheel to point of initial contact, then add 1/2 turn of the wheel.
Microseal ‘B’ Adhesive Seals
MSB-1001 100 sheets per package
Related Product
MSR-0001 Sealing roller, quickly and uniformly presses seal over plate wells
Sealing Comparison Chart
Product Product Highlights Compatibility
Microseal ‘B’
adhesive seals
MSB-1001
(100 sheets)
• Clear adhesive seal for thermal cycling or fluorescent assays
• Plate storage or transport before or after cycling
(ambient to –40°C)
• PCR volumes ≥10 µl (96-well) or ≥5 µl (384-well)
All polypropylene
PCR plates
Microseal ‘A’ film
MSA-5001
(50 sheets)
• Smooth application and release — no aerosol generation
• Peel-away release liner for pristine sealing surface
• PCR volumes ≥10 µl (96-well) or ≥5 µl (384-well)
All PCR plates,
tube strips
Microseal ‘F’ foil
MSF-1001
(100 sheets)
• Foil can be pierced for sample recovery
• Plate storage (ambient to –70°C)
• PCR volumes ≥25 µl (96-well) or ≥5 µl (384-well)
All PCR plates
96-well PCR plate
sealing mats
223-9442 (5 mats)
• Reusable mat — ideal for sequencing
• Smooth release — no aerosol generation
• PCR volumes ≥10 µl
96-well polypropylene
PCR plates
Microseal ‘P’ and
‘P+’ pads
MSP-1001
MSP-1002
(10 pads)
• Reusable sealing pad adheres to the heated lid
• Use MSP-1001 with Power Bonnet
™
motorized lid
(ALP-1296, ALP-1238), using PCR volumes ≥15 µl (96-well)
or ≥10 µl (384-well)
• Use MSP-1002 with Moto Alpha
™
motorized lid
(ALP-2296, ALP-2238), using PCR volumes ≥10 µl (96-well)
or ≥5 µl (384-well)
All 384-well and
96-well PCR plates
with raised well rims


