M7318160G.qxd 05/08/2003 2:04 PM Page i Model EM-1 Econo UV Monitor Instruction Manual Catalog Numbers 731-8160 731-8162 For Technical Service Call Your Local Bio-Rad Office or in the U.S.
M7318160G.qxd 05/08/2003 2:04 PM Page ii Warranty Model _________________________________________ Serial Number __________________________________ Date of Delivery_________________________________ Warranty Period _________________________________ Unless otherwise specified, instruments sold by Bio-Rad Laboratories are under warranty for 1 year against defects in materials and workmanship. If any defects should occur during this warranty period, Bio-Rad will replace the defective parts without charge.
M7318160G.qxd 05/08/2003 2:04 PM Page iii Table of Contents Section 1 Safety.......................................................................... 1 Section 2 Introduction............................................................... 2 Section 3 Unpacking.................................................................. 2 Section 4 Description and Features ......................................... 4 4.1 4.2 4.3 Front Panel Functions .........................................................
M7318160G.qxd 05/08/2003 2:04 PM Page 1 Section 1 Safety ! Disconnect supply before servicing. No user serviceable parts inside. Refer servicing to Bio-Rad service personnel. ! Disconnect supply before removing optics module connector. ! Optics module produces ultraviolet radiation. Disconnect supply before changing filter or flow cell. This instrument is intended for laboratory use only.
M7318160G.qxd 05/08/2003 2:04 PM Page 2 Section 2 Introduction Fig. 2.1. Model EM-1 Econo UV Monitor. The Model EM-1 Econo UV Monitor is a high quality dual wavelength detector for liquid chromatography. The monitor consists of a control unit and a portable optics module. The optics module comes equipped with two interchangeable filters (280 nm and 254 nm), a userserviceable mercury lamp, and a 2 mm pathlength flow cell.
M7318160G.qxd 05/08/2003 2:04 PM Page 3 Cable #4 Optics Module (4) (4) (4) (2) Fittings Control Unit Power Cord Also includes: Instruction Manual Fig. 3.1. Parts supplied with the Model EM-1 Econo UV Monitor.
M7318160G.qxd 05/08/2003 2:05 PM Page 4 Section 4 Description and Features The Model EM-1 Econo UV Monitor consists of two units. The control unit houses the controls and the majority of the electronics, while the optics unit contains the UV lamp, the flow cell, and filter tray, which contains both 254 nm and 280 nm filters. The following tables and illustrations describe the features of the Model EM-1 Econo UV Monitor.
M7318160G.qxd 05/08/2003 2:05 PM Page 5 Display Function Range Selector Allows the user to select one of eight range settings between 0.01 and 2.00 AUFS (Absorbance Units Full Scale). Level Indicator This bar graph is a convenient graphic representation of the % AUFS. Each green segment equals 10% full scale. Auto-Zero Sets output voltage to 0.00 and defines that as the baseline. (A maximum of 0.25 AUFS can be zeroed out.) Event Mark Causes a mark to be superimposed on the recorder trace.
M7318160G.qxd 05/08/2003 2:05 PM Page 6 4.2 Rear Panel Sockets Fig. 4.2. Rear panel sockets. The rear panel of the Model EM-1 Econo UV Monitor control unit contains four sockets for electrical connections (see Figure 4.2). Below is a brief description of each socket. For a more technical description of each socket, see Appendix C. Socket Function Power Entry Module IEC power entry module with four-position international voltage selector. The monitor is shipped in its 120 V or 220 V version.
M7318160G.qxd 05/08/2003 2:05 PM Page 7 Socket Function Auxiliary Socket Input/output to allow the UV monitor to be used with equipment other than the Bio-Rad components. See Section 7 and Appendix C. 4.3 Optics Module Rack Mount Filter Tray Lamp Housing Flow Cell Fluid In/Out Ports (Note arrows on the top side) Power/Signal Cord Fig. 4.3. The optics module. Filter Tray Fluid In/Out Ports Rack Mount Power/Signal Cord Lamp Housing Flow Cell Compartment Contains filters for 254 nm and 280 nm.
M7318160G.qxd 05/08/2003 2:05 PM Page 8 Section 5 Setting Up 5.1 Voltage Conversion Warning: The Model EM-1 Econo UV Monitor is shipped in its 120 V or 220 V version. To operate at other voltages, refer to the procedure below. Failure to follow this procedure may result in damage to the unit and invalidation of the warranty. Prior to connecting the power cord to the power entry module and the mains outlet, make sure that the voltage setting showing on the power entry module matches your line voltage.
M7318160G.qxd 05/08/2003 2:05 PM Page 9 5.2 Wavelength Selection The Model EM-1 Optics Module Assembly is shipped with a filter tray containing filters for both 254 nm and 280 nm (note markings on the filter unit). It is shipped in the 280 nm configuration. To change from one wavelength to another, loosen the retaining screw and remove the filter tray. Rotate the tray 180Þ and reinsert (do not over-tighten the thumb screw). The light path is indicated by the ³ on the bottom of the case. Fig. 5.2.
M7318160G.qxd 05/08/2003 2:05 PM Page 10 5.4 Connecting the Model EM-1 Econo UV Monitor to Recording Equipment The Model EM-1 Econo UV Monitor is shipped with a cable (System Cable 4) that allows connection of the monitor to devices that use banana plug connectors. System Cable 2 is available separately to connect the Model EM-1 Econo UV Monitor to the Model 1327 Chart Recorder. Following is a description of connections to recording instruments.
M7318160G.qxd 05/08/2003 2:05 PM Page 11 When the light stops flashing and remains lit, the Model EM-1 Econo UV Monitor is ready for use. Wait at least 20 minutes if an AUFS setting of 0.05 or less is to be used. Note: If the Model EM-1 Econo UV Monitor is used in a cold room or cold box, leave the lamp on continuously to prevent the formation of condensation. 3. Start the flow of buffer or solvent, and continue for several minutes prior to starting a separation or measurement. 4.
M7318160G.qxd 05/08/2003 2:05 PM Page 12 Avoid letting the flow cell become dry, as dissolved solids may precipitate and attach to the walls. This will result in baseline drift and possibly a noisy signal. If the flow cell becomes dirty, it should be cleaned with an appropriate solvent. (SDS, 1M HCl, 1M NaOH, ethanol, acetone, and methylene chloride will not harm Model EM-1 Econo UV Monitor flow cells.) If NaOH is used, it should not be left in the flow cell for more than 20 minutes.
M7318160G.qxd 05/08/2003 2:05 PM Problem Page 13 Possible cause Lamp will not Optics module not light,or lamp LED plugged in on continues blinking tor. Chart recorder not responding No power Solution Plug optics module into the socket rear of UV moni- Lamp burned out Replace lamp (see Appendix B). Chart recorder is plugged into AUX socket Plug chart recorder into the socket. Full scale of recorder set too high Set recorder range to 1 V. Recorder has bottomed out Readjust pen position.
M7318160G.qxd 05/08/2003 2:05 PM Page 14 Appendix A Flow Cell Replacement 1. Remove the retaining clip from the bottom of the optics module and pull the luer connectors away from their holders as shown in Figure A-1. 2. Unscrew thumbscrew and remove the old flow cell. 3. Reinsert the new flow cell following the above steps in reverse order. (Do not over-tighten the thumb screw.) ! Warning: To avoid exposure to UV radiation, make sure that the UV lamp is off when changing filters and flow cells.
M7318160G.qxd 05/08/2003 2:05 PM Page 15 Appendix B UV Lamp Replacement The coated low pressure mercury lamp has an expected lifetime of at least 2,000 hours. To replace a lamp, proceed as follows, referring to Figure B-1: 1. Unplug the optics module from the rear panel of the control unit. ! Warning: Failure to unplug the optics module from the control unit may expose the user to high voltage and UV radiation. 2.
M7318160G.qxd 05/08/2003 2:05 PM Page 16 Filter Tray UV Lamp Lamp Holder Lamp Connector Fig. B-1. UV lamp replacement.
M7318160G.qxd 05/08/2003 2:06 PM Page 17 Appendix C Rear Panel Connections Both the signal output and the AUX sockets are 8-pin circular mini-DINs. The following information is provided for those wishing to interface the Model EM-1 Econo UV Monitor with other equipment such as integrators and non Bio-Rad fraction collectors. System Cable 7 may be used as a breakout cable, with an 8-pin mini-DIN at one end and loose wires at the other.
M7318160G.qxd 05/08/2003 2:06 PM Page 18 Appendix D Technical Specifications Wave length Operating mode Lamp 254 nm and 280 nm AU Low pressure mercury lamp with phosphor screen Filters Interference type 280 nm 254 nm Operating temperature +4° to 40° C Baseline offset ± 0.25 AU Auto-Zero Time constant 1.5 sec to 90% of full response at all ranges Sensitivity ranges 2.0, 1.0, 0.5, 0.2, 0.1, 0.05, 0.02, 0.
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M7318160G.qxd 05/08/2003 2:06 PM Page 20 Bio-Rad Laboratories Life Science Group Bulletin 0000 US/EG Web site www.bio-rad.
