M7318150H 2/27/1 2:05 PM Page i Model EG-1 Econo Gradient Monitor Instruction Manual Catalog Numbers 731-8151 731-8150
M7318150H 2/27/1 2:05 PM Page ii Warranty Model _________________________________________ Serial Number __________________________________ Date of Delivery_________________________________ Warranty Period _________________________________ Unless otherwise specified, instruments sold by Bio-Rad are under warranty for 1 year against defects in materials and workmanship. If any defects should occur during this warranty period, Bio-Rad will replace the defective parts without charge.
M7318150H 2/27/1 2:05 PM Page iii Table of Contents Section 1 Safety............................................................................. 1 Section 2 Introduction.................................................................. 2 Section 3 Unpacking..................................................................... 3 Section 4 Description and Features ............................................ 4 4.1 4.2 4.3 Front Panel Functions..................................................
M7318150H 2/27/1 2:05 PM Page 1 Section 1 Safety ! Disconnect supply before servicing. No user serviceable parts inside. Refer servicing to Bio-Rad service personnel. This instrument is intended for laboratory use only. This product conforms to the “Class A” standards for electromagnetic emissions intended for laboratory equipment applications. It is possible that emissions from this product may interfere with some sensitive appliances when placed nearby or in the same circuit as those appliances.
M7318150H 2/27/1 2:06 PM Page 2 Section 2 Introduction Fig. 1.1. Model EG-1 Econo Gradient Monitor. The Model EG-1 Econo Gradient Monitor is a high quality, easy to use, conductivity and gradient monitor for liquid chromatography. The monitor consists of a control unit and an external flow cell. The flow cell is fitted with luer connectors for rapid tubing connections. It can be connected to the outlet of an Econo-Column® chromatography column or an Econo-Pac® Cartridge.
M7318150H 2/27/1 2:06 PM Page 3 Section 3 Unpacking Carefully remove the contents of the shipping box, and check for any obvious damage or problems with the instrument. Figure 2.1 shows the parts included with the Model EG-1 Econo Gradient Monitor. Check off all parts against the supplied packing list. If any parts are missing or damaged, contact Bio-Rad Laboratories immediately. Flow Cell Cable 4 Control Unit Also includes: Instruction Manual, 3 male luer fittings and 3 female luer fittings. Fig.
M7318150H 2/27/1 2:06 PM Page 4 Section 4 Description and Features The Model EG-1 Econo Gradient Monitor consists of a control unit and a flow cell. The control unit houses the electronics and user interface. The flow cell connects to the control unit and is external for flexible placement. The following tables and illustrations describe the features of the Model EG-1 Econo Gradient Monitor. 4.1 Front Panel Functions Fig. 3.1. Front panel controls.
M7318150H 2/27/1 2:06 PM Page 5 Display Function Bar Graph In the Conductivity mode, the Bar Graph provides a graphic representation of the buffer conductivity relative to the lower and upper limits of the selected range. In the Gradient mode, the Bar Graph provides an indication of the buffer conductivity relative to buffer A and B. A flashing red LED at either end of the Bar Graph indicates an out of range condition. Mode Selector Allows selection of Conductivity or Gradient mode.
M7318150H 2/27/1 2:06 PM Page 6 4.2 Rear Panel Sockets Fig. 3.2. Rear panel sockets. The rear panel of the Model EG-1 Econo Gradient Monitor control unit contains an On/Off power switch and four sockets for electrical connections (see Figure 3.2). A brief description of each socket follows. Display Function Power Entry For connection of the 12 VDC power adaptor.
M7318150H 2/27/1 2:06 PM Page 7 4.3 Flow Cell Fig. 3.3. The flow cell. Inlet/Outlet Ports A male and a female luer fitting on either end of the flow cell allow rapid tubing connection. The flow cell can be used with flow in either direction. Note: the luer fittings may be unscrewed and replaced with 1/4”-28 threaded flat-bottom fittings. Signal Cable For connection of the flow cell to the the rear panel of the control unit. socket on Section 5 Setting Up 5.
M7318150H 2/27/1 2:06 PM Page 8 Model 1327 Econo Recorder. The cable also connects the Econo Gradient Monitor to other recording devices which use banana plug connectors. Connecting the Model EG-1 Econo Gradient Monitor to a Model 1327 (dual pen) Econo Recorder The Model EG-1 Econo Gradient Monitor must be connected to channel 2 of the Model 1327 Econo Recorder. Use System Cable 4 to connect the Econo Gradient Monitor to the banana plug signal inputs on the rear panel of the Econo Recorder.
M7318150H 2/27/1 2:06 PM Page 9 6.1 Conductivity Mode In the Conductivity mode, the numerical LED displays the conductivity (in Siemens) of the solution in the flow cell, and the LED indicator light indicates the range selected. The Bar Graph gives an indication of the conductivity relative to the range setting currently chosen (thereby giving an indication of the relative position of the chart recorder pen). 1.
M7318150H 2/27/1 2:06 PM Page 10 4. Pass buffer B through the flow cell until the conductivity reading stabilizes. (A flashing CAL indicates the monitor is calibrating.) 5. Press the CAL key to accept the conductivity value for buffer B. Note: If the 3-digit display reads “Err”, the conductivity valves for buffer A and B are too close together to use the Gradient mode. Press the Mode Selector key to cancel calibration and use the Conductivity mode.
M7318150H 2/27/1 2:06 PM Page 11 Model EG-1 Econo Gradient Monitor Control Unit and Flow Cell: The control unit may be left on continually (power consumption is negligible). Although the cases are chemical resistant, spills and splashes may cause precipitates to form on the component cases. Use a squirt bottle and soapy water to wash down the outer cases of the instrument. Flow Cell Clean the outside surface of the flow cell as described above.
M7318150H 2/27/1 2:06 PM Page 12 Problem Possible Cause Larger or smaller than Chart recorder input expected gradient voltage set incorrectly profile Ghost peaks appear in gradient profile Chart recorder not responding Solution Check chart recorder settings (See Sections 4.2 and 5) If using Gradient mode, buffer A and B values not set correctly Re-calibrate the gradient mode Charged sample detected (e.g. protein) No problem Air bubbles passing through flow cell Use degassed buffers.
M7318150H 2/27/1 2:06 PM Page 13 Appendix A Fuse Replacement Figure A. Fuse removal. 1. Disconnect unit from power source. 2. Remove the fuse cover with a small-blade screwdriver or similar tool. 3. Pull the fuse holder out of the fuse compartment and, if necessary, replace the fuse. Use 5 mm x 20 mm fuse, type T, 1A. 4. Reinsert the fuse holder, with fuse, into the fuse compartment. Appendix B Rear Panel Connections The signal output socket is an 8-pin circular mini-DIN.
M7318150H 2/27/1 2:06 PM Page 14 Appendix C Technical Specifications General Operating mode Conductivity, in units of Siemens (See Section 1) Operating temperature 4-40 °C Sensitivity ranges (full scale) 0-10µS, 0-100µS, 0-1000µS, 0-10mS Output signal 0-1 Volt Power requirements 90-264 V, 50-60 Hz (depending upon power adaptor used) Power consumption 20W maximum Noise Short Term: < 0.1 µS Long Term: < 0.5 µS Linearity +/-0.5 % full scale Accuracy +/-2 % full scale Drift < 0.
M7318150H 2/27/1 2:06 PM Page 15 Appendix D Ordering Information Model EG-1 Econo Gradient Monitor 731-8151 Econo Gradient Monitor, Model EG-1, with 100-120 V power adaptor (catalog number 731-8270) and flow cell (catalog number 731-8155) 731-8150 Econo Gradient Monitor, Model EG-1, requires power adaptor, includes flow cell (catalog number 731-8155) 731-8155 Flow Cell, Model EG-1, 1 cell 731-8270 Power Adaptor, 100-120 V, for USA, Canada, Japan, Mexico, Taiwan, Latin America 731-8271 Power Ada
M7318150H 2/27/1 2:06 PM Page 16 Bio-Rad Laboratories Life Science Group Bulletin 0000 US/EG Web site www.bio-rad.
