Model 680 Microplate Reader Instruction Manual Catalog Numbers 168-1000 168-1001 168-1002 168-1003
Warranty Statement This warranty may vary outside of the continental United States. Contact your local Bio-Rad office for exact terms of your warranty. Bio-Rad Laboratories warrants to the customer that the Model 680 Microplate Reader (catalog number 168-1000, 168-1001, 168-1002, and 168-1003) will be free from defects in material and workmanship, and will meet all performance specification for period of one year from the date of shipment. This warranty covers all parts and labor.
Regulatory Notices Electro Magnetic Compatibility: This instrument is designed to meet the Class-A emissions and immunity levels of the EN61326 product family standard for the CE mark. This rating requires that it only be used in a laboratory environment. Note: This instrument has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC rules.
Table of Contents Section 1. ........Introduction ................................................................................................ 1 1.1 Description and accessories for Model 680 Microplate Reader ..........................2 Section 2. ........Product Description ..................................................................................... 3 2.1 Contents of Shipping Carton ..........................................................................3 2.2 External Features...............
Section 1. Introduction The Model 680 Microplate Reader is an eight-channel, vertical pathlength photometer that measures the absorbance of the contents in the wells of a 96-well microtitration plates. It can perform single or dual wavelength measurements and can report absorbance values to three decimal places. The Model 680 reader can be programmed by entering commands through the membrane keypad to define plate reading and data analysis parameters as testing protocols and select report types.
1.
Section 2. 2.1 Product Description Contents of Shipping Carton The shipping carton contains the following items: • Model 680 Microplate Reader, with 415nm, 450nm, 490nm and 655nm interference filters installed on the filter wheel. • Power cord • Serial PC Cable • Spare fuse • Dust cover • Instruction manual • Warranty card • One roll of thermal printer paper (for units equipped with internal printer) Inspect the exterior of the instrument for any signs of shipping damage.
2.2 External Features 4 1 5 2 3 6 1. Release latch for rear cover 2. Liquid crystal display (LCD) 3. Membrane keypad 4. Rear cover 5. Printer slot (when optional built-in printer is installed) 6. Reading chamber door 7 8 14 11 10 9 7. RS-232C serial interface 8. Serial number Label 9. Fuse 10. Power switch 12 13 11. Printer port (only when optional built-in printer is not installed) 12. Power cord receptacle 13. Cooling Fan 14.
2.3 Membrane Keypad 13 1 2 Sta rt/ Sto p P ap er Feed P rin t 5 Ch ange 12 11 7 CP 8 CN 9 CW 4 CO 5 QC 6 CAL 1 SMP 2 BLK 3 S TD 0 EMP . FUNC A⇒ Z ＋ －・・ Back E dit Ma in 3 4 Memo ry Recall 10 Z ⇒A 6 1. Main 2. Start/Stop 3. Paper Feed 4. Up Arrow 5. Left Arrow 6. Down Arrow 7. Right Arrow 8. Dot/Function 9. Enter 10. Ten Keys 11. Print 12. Edit 13. Memory Recall 8 7 9 Returns to MAIN SCREEN. Initiates plate reading using current active protocol.
Section 3. 3.1 Instrument Set-up Initial Start-up 1 Place the instrument on a clean, sturdy table or bench. It is important to keep the instrument in a clean, relatively dust free environment to ensure optimal performance. 2 Connect the power cord to the back of the instrument. Before connecting the instrument to the main electrical supply, check that the AC voltage is appropriate for the instrument. 3 Turn on the power switch on the rear panel.
Section 4. 4.1 Operation Instrument Overview The Model 680 Microplate Reader has built-in software that allows the user to set the plate reading and data analysis conditions as a protocol data, read a microplate under the defined reading conditions, and get the printout reports from the internal or external printer. The software communicates through the 4-line, 20-character LCD and is controlled through the instrument's membrane keypad. The display shows the current mode of the on-board software.
Operation flow when End point protocol selected Not Use Edit Constant Ranged Cutoff Single Cutoff Cutoff Values setting Gray zone setting when single Cutoff Control Ranged Cutoff Single Cutoff Cutoff Control setting Gray zone setting when single Cutoff Formula 12 choices of cutoff formula Coefficient setting of selected formula Cutoff Ratio Concentration 0 - 9999 17 choices of unit Unit Ranged Cutoff Single Cutoff Cutoff value Protocol Report Raw(Raw data), Abs(Absorbance), Lim(Limit), Mt
Operation flow when Kinetic protocol selected Edit Reading Inter Shaking Ph.mode Speed Incu Mode Number of reads (2-30), Start delay time (0-999) Reading interval time Shake (First/Every/disable), Speed (High, Low, Mid), Time Single / Dual Wavelength Read Speed (Fast/Step) Disable/Enable Temperature CC Limit (correlation coefficient limit) Calc.
4.2 Functions Overview 4.2.1 Calculations The Microplate Reader uses Beer's Law to calculate the absorbance value of each well. Beer's Law states that absorbance is equal to the log10 of the ratio of the baseline measurement intensity (Io) to the sample measurement intensity (I). Beer's Law Absorbance = Log10 (Io/I) Before measuring the plate, the reader takes a reading for all eight photodiode channels. These values are recorded as the baseline measurement (Io) values for each channel, respectively.
in this memory. The first time the instrument is turned on, or after a battery failure, the following default information will be held in the memory. 1. Both of Administrator and Common user security password are set to "00000". 2. Laboratory name is "Bio-Rad Laboratories". 3. All wavelength values of the filters are set to "---" nm. 4. The date and time of the calendar chip are set to 1 day, 1 month, 00 year, 0 hour and 0 minute. 5. None of plate reading data is available. 6.
Blank mean = X/n S.D. = [{X^2 – n*(Blank mean)^2}/{n-1}]^1/2 Where: S.D. = Standard deviation X = Sum total of the raw absorbance for each blank X^2 = Sum total of the squared raw absorbance for each blank n = Number of blanks (3) Limit report The Limit report provides a qualitative YES/NO report.
Upper cutoff absorbance = Positive absorbance + ((Gray zone/100) * Positive absorbance) Lower cutoff absorbance = Positive absorbance – ((Gray zone/100) * Positive absorbance) For units not designated "Abs", the absorbance value of each well is converted to the concentration value using the curve fit constants of the curve fit report. If the concentration of a well is within the gray zone value of the positive cutoff value, the well is scored "*".
The result absorbance value of the formula calculation and the gray zone value that is entered by the user are used for the cutoff. The upper and lower cutoff values are: Upper cutoff absorbance = Result of a Formula + ((Gray zone/100) * Result of a Formula) Lower cutoff absorbance = Result of a Formula - ((Gray zone/100) * Result of a Formula) The absorbance range of the upper and lower cutoff is divided into 10 equal partitions, numbered 0 through 9.
i. ii. iii. iv. v. vi. vii. viii. ix. x. 5P Logistic Rodbard 4P Logistic Rodbard 5P Logistic Cook-Wilkenson 4P Logistic Cook-Wilkenson 5P Exponential Sigmoid Logistic Linear regression Quadratic regression Cubic spline Point To Point regression The onboard software calculates the best fit of the defined curve between each set of two consecutive data points in the standard curve of absorbance vs. concentration.
Negative cutoff = (Negative Control average + 0.210) * 0.900 TeSeE eq: Positive cutoff = Negative Control average + 0.210 Negative cutoff = Negative Control average + 0.090 Samples are interpreted as follow: “POS”: Positive when sample OD >= Positive cutoff “NEG”: Negative when sample OD < Negative cutoff “???”: Gray zone: NEG cutoff =< sample OD < POS cutoff Reports of Kinetic Protocol (1) Absorbance report The Kinetic Absorbance report is the series of absorbance data in the kinetic sequence.
4.2.7 Printer The Model 680 Microplate Reader has a built-in printer interface connector (25 pin D-sub) or an optional factory built-in printer. The printer interface connector allows the user to connect an external printer for the printout of reports or protocol. This external printer interface is based upon Centronics interface standards. Two types of printer, ASCII type and ESC/P type, are allowable to connect.
4.3 Detailed Operation 4.3.1. Initial Start-up Self-diagnosis Model 680 Microplate Reader 13:05 18/09/01 Initializing reader Self-Diagnosis This screen will appear upon power-up and during self-diagnosis. Date and Time shall also be indicated on this screen. Initialization After self-diagnosis, the system shall initialize all the hardware. Login SCREEN System Login User: Administrator Password:***** Press ENTER MAIN SCREEN 01:EndPoint Assay01 M405(1)R655(6) Shake:999s,Mid 38.2/40.
4.3.2. Configuring the system Note: To return to the previous screen, use the back/left arrow key. Edit Menu »■Protocol •Lab.name •Security •SaveSTDC •Filters •Printer •Date set Edit Menu This menu can be accessed by pressing the Edit key in the MAIN SCREEN. Security Menu Security function »■Change Password •Change user •Lock/Unlock Prtl Security Setting Select “Security” in Edit Menu screen above, and press Enter. Then Security Menu shall be on the screen.
Filter Filter setting 1:400nm 2:450nm 3:475nm 4:650nm 5:650nm 6:...nm 7:...nm 8:...nm Move the cursor to the desired position and using the Up, Down, and Right arrow keys. Enter the filter wavelength on the selected position using the number keys. If the selected position of the filter disk is empty, enter three “...”. Press Enter to complete the field entry. The system will return to the previous Edit Menu screen.
External Printer setting Printer Select ext printer type > [ASCII] ESC/P Select “Printer” in Edit Menu screen above, and press Enter. Then, Select ext printer type menu shall be appeared on the screen. The Up and Down arrow keys are used to move the cursor to the desired printer; “ASCII” type or “ESC/P” type. Pressing Enter accepts the setting and the reader returns to the Edit Menu screen.
4.3.4. Memory Recall operation MEMORY RECALL MENU Memory Recall Menu Memory Recall »■Protocol •Plate Data This menu screen can be accessed by pressing the Memory Recall key in the MAIN screen. Protocol type menu Select protocol type »■End Point •Kinetic •Checkmark Changing current active protocol Select “Protocol” in Memory Recall menu above, and press Enter. The Select Protocol type menu will appear on the screen. Select a protocol using the Up and Down arrow keys.
4.3.5. Editing Kinetic protocol Edit Menu Edit Menu »■Protocol ·Lab.name ·Security •SaveSTDC ·Filters •Printer ·Date set This menu screen can be accessed by pressing the Edit key in the MAIN screen. Kinetic Protocol Menu »■Mode ·Report ·Calc. ·Kit name ·Mapping ·Assay Kinetic Protocol Menu Select “Protocol” in the Edit Menu and press Enter. The Kinetic Protocol Menu will appear on the screen, if the current active protocol type is Kinetic mode. KINETIC Mode »■Reading ·Inter. ·Shaking ·Ph.
Speed Reading speed [Fast mode] Step mode Incubation setting Set Incubation param Incubator: Enable Temperature: 37.5 Selecting Reading Speed Pressing the Up or Down arrow keys will switch the selection. Press Enter to complete the selection. Incubator parameter setting When the “Incubator” field is activated, pressing the Right arrow key will switch the field entry: “Enable” <-> ”Disable”. Pressing the Up or Down arrow keys will move the cursor to the next entry field.
4.3.6. Checkmark value setting (by Administrator only) Edit Menu Edit Menu »■Protocol •Lab.name •Security •SaveSTDC •Filters •Printer •Date set This menu screen can be accessed by pressing the Edit key in the MAIN screen. Checkmark Protocol Menu Checkmark Protocol Menu »■Serial #/Report •Linearity ref. •Max.CV% •Filter Check ref. Select “Protocol” in the Edit Menu and press Enter. The Checkmark Protocol Menu will appear on the screen, if the current active protocol type is Checkmark mode.
4.3.7. Editing End Point Protocol Edit Menu »■Protocol •Lab.name •Security •SaveSTDC •Filters •Printer •Date set Edit Menu This menu screen can be accessed by pressing the Edit key in the MAIN screen. End Point Protocol Menu »■Cutoff •Report •Limit •STDs End Point Protocol Menu •Mode •Mapping •Kit name Select “Protocol” in Edit Menu screen above and press Enter. The End point Protocol Menu will appear on the screen, if the current active protocol type is End point mode.
4.3.8. Cutoff setting procedure »■Not usex [Const] •Contrl •Formula •Ratio Cutoff setting Menu This menu is entered by choosing "Cutoff" in the End Point Protocol Menu. It is used to set the cutoff type. The cutoff type marked with “[ ]” is the current selection. The Up and Down arrow key moves the cursor to the desired item in the menu list. When the desired parameters have been selected, press Enter twice to accept the selection and to resume to the next screen.
b. Cutoff Control setting »■Ranged cutoff [Single cutoff] Selection of Single/Ranged Cutoff Control This menu screen selects Single or Ranged cutoff type. The cutoff type marked with “[ ]” is the current selection. The Enter key seals the selection and resumes to the next setting screen. Single cutoff Positive >= CNx Gray zone: 50% Unit: Abs Ranged Positive >= CPx Negative < CNx Unit: Abs Enter key to accept Single Cutoff Control The Numerical keys are used to enter the gray zone value.
c. Cutoff Formula setting Select formula »■[k*CNx •k*CPx ↓ •k*COx ↑ »■CNx •CPx •COx ↓ •k + Selection of Formula ] / k / k / k CNx ↑ »■k + CPx •k + COx •k*CNx + CPx ↓ •(CNx + CPx)/ k Page-1 Page-2 Page-3 ↑ »■k1*CNx + k2*CPx Page-4 k * CNx k * CPx k * COx CNx / k CPx / k COx / k k + CNx k + CPx k + COx k * CNx + CPx (CNx + CPx) / k This menu screen selects a formula for cutoff calculation. The formula marked with “[ ]” is the current selection.
d. Cutoff Ratio setting Ratio to Calibrator »■Concentration •Unit •Cutoff value Concentration Unit Cutoff value Ratio to Calibrator In this screen, there are three items for setting the ratio to calibrator. The Up and Down arrow key moves the cursor to the desired item in the menu list. The Enter key seals the selection and resumes to the next setting screen.
4.3.9. Standard setting procedure Standard curve setting Menu Standard menu »■STD information •Curve fit •Recall STD curve This menu screen is entered by choosing "STDs" in the End Point Protocol Menu screen. It is used to set the concentration data and the curve fit type for a standard curve or to recall a memorized standard curve. The Up and Down arrow key moves the cursor to the desired item in the menu list. The Enter key seals the selection and resumes to the next setting screen. a.
b. Curve fit setting Curve fit Curve fit setting »■Curve fit type •Graph axis Curve fit type In this screen, there are two items for setting the curve fit. The Up and Down arrow key moves the cursor to the desired item in the menu list. The Enter key seals the selection and resumes to the next setting screen. ↑ 5p »■ 4p [5p ↓ 4p Curve fit type logisticRodb logisticRodb logisticCook] logisticCook 10 types of curve fit are supported as below.
c. Recall STD curve setting Recall STD curve »■Recall setting •Select STD curve Recall Setting of Standard Curve In this screen, there are two items for setting the Recall STD curve as below. The Up and Down arrow key moves the cursor to the desired item in the menu list. 1. Recall setting (Yes/No) 2. Selecting the storage number of standard curves (1 -> 5) Recall setting Recall setting Recall setting »■Yes No Move the cursor to the desired position using the Up, Down or Right arrow keys.
4.3.10. Mode setting procedure »■Set Photo mode •Set shaking •Set read mode •Incubation Mode setting Menu This menu screen is entered by choosing the "Mode" in the End Point Protocol Menu screen or the Kinetic Protocol Menu screen. It is used to set the plate reading conditions. The Up and Down arrow key moves the cursor to the desired item in the menu list. The Enter key seals the selection and resumes to the next setting screen. a.
d. Incubation Set Incubation param Incubator: Enable Temperature: 37.0 Incubation In this screen, the user switches on/off the Incubator and sets the incubation temperature. The temperature setting range is from 25.0 to 45.0 degrees. The Up and Down arrow key moves the cursor to the desired parameter. The Right arrow key chooses “Enable” or ”Disable” (ON/OFF) of Incubator. The Numerical keys and the Decimal point key are used to input the incubation temperature.
4.3.11. Plate Map setting procedure Select Mapping mode Menu Select Mapping mode »■Manual mapping •Automatic mapping This menu screen is entered by choosing the "Mapping" in the End Point Protocol Menu screen or the Kinetic Protocol Menu screen. It is used to set the plate format (plate mapping). The Up and Down arrow key moves the cursor next to the desired item of menu list. The Enter key seals the selection and changes this screen to the next setting screen. a.
Acceptable range of number for each well type: 1. 2. 3. 4. Blank and Empty wells are not acceptable for any number. Sample and Standard wells: 00 - 99 Standard wells: 01 - 99 Control and Calibrator wells: 0-9 b. Automatic mapping Automatic mapping Automatic BLK= 1 CP CN = 1 CO CW = 0 QC mapping = 1 CAL= 0 = 0 SMP=93 = 0 REP= 1 Plate auto-mapping completed In this screen, the user can input each number of the well types for a plate map. These defined numbers are used to set a plate map automatically.
Warning and Error of Automatic Mapping: Warning: The total Of mapped wells has Exceeded 96. Press any key Warning screen: If the total number of mapping wells exceeds 96 wells, a warning message in the left figure will appear on the screen. The calculation of the total number of mapping wells is as below: (Blanks+Standards+CNs+CWs+CPs+COs+QCs+Calibrators+Sample)*Replicates The plate mapping is executed normally and the exceeded part is invalidated.
4.4 Quick Guide – Reading a plate 1. Turn on the instrument. Allow about 15 seconds for the self-diagnosis. Allow the instrument to warm up for about three minutes prior to reading a plate. 2. Upon power up, the login screen will appear. Enter the login password (initial password: 00000), press Enter. 3. Program a protocol for your assay. See section 4.3.5 for editing a Kinetic protocol, or section 4.3.7 for editing an End point protocol.
4.5 Language choice The Model 680 Microplate Reader has two versions, English and Chinese, in the on-board software. And it allows the user to choose between English and Chinese. 1. Language choice screen Press both “0” and “4” key and turn the power on, then hold its key pressing. About 5 seconds Select Language 1. English 2. Chinese ENTER 0.
(5). Retrieval function for plate data The Chinese version does not support this feature. The URPD (Upload Raw Plate Data) and DRPD (Download Raw Plate Data) command in Command Language does not function in Chinese version. (6). Serial number input/output The Chinese version does not support Serial number input/output. It means that the serial number does not appear in the head line of each printout report.
Section 5. Instrument service by the user Caution: Electrical shock hazardous! Always disconnect the instrument from the AC power source before opening the service compartment. Caution: The lamp and the lamp cover may be very HOT ! Use caution and allow the lamp to cool before attempting service. 5.1 1. 2. 3. 4. 5. 5.2 1. 2. 3. 4. 5. 6. 7. 8. 9.
Section 6. Troubleshooting and Error Messages The Model 680 Microplate Reader constantly monitors several functions and will display appropriate error message on the LCD when errors are encountered. 1. 2. 3. LCD appears blank on power up. a. Power switch is not turned on. Turn on power switch on the rear panel. b. Unit is not plugged into AC outlet or power code is not attached to the instrument. Check power cord connections at outlet and on the rear panel of the instrument. c.
7. LCD displays “Filter not installed”. 8. Wells have color, but absorbance values seem low. Enter the Edit Menu by pressing Edit key, and set up the filters. a. Incorrect filter used for measurement and /or reference wavelength. Check wavelength used in analysis. Read plate in single wavelength mode at all wavelength to verify that the proper filter was used. The measurement wavelength should produce the highest absorbance. b.
Section 7. 7.
Accuracy ±1.0% or 0.010 from 0 to 3.0 OD at 490nm Linearity ±1.0% from 0 to 2.0 OD, ±2.0% from 0 to 3.0 OD Reproducibility 1.0% or 0.005 OD from 0 to 2.0 OD, 1.5% from 2.0 to 3.0 OD Inter-channel variation 1.5% or 0.005 OD from 0 to 3.0 OD Stability and Drift 0.010 OD at OD=1 with single wavelength reading Incubator (Factory option) Temperature set point 25ºC to 45ºC, 0.1ºC step Set point must be 5ºC above ambient. Set point accuracy Less than ±0.
7.2 Interface Specifications The following specification describes the syntax and language required for computer control of the reader. 1. SIGNALS TXD: RXD: DTR: DSR: CTS: RTS: transmit data receive data data terminal ready data set ready clear to send request to send 2. TRANSMISSON Baud rate: 9600 Data width: 8 Stop width: 1 Parity : None 3. DATA All data must be transmitted in ASCII. The READER command interpreter must be “case blind” (no distinction between upper or lower case ASCII characters).
7.3 Command Language for Model 680 Microplate Reader 7.3.1 Command Language for Remote control mode 1-1. Syntax SYNTAX: Command: Response: [ ... ] : "EIA.READER" – Model680 reader [ ... ] : “ERE” 1-2. Commands 1-2-1.
1-2-6 RM (Reset maintenance report) : : : "RM" Reset maintenance report values. none "ERE" 1-2-7. ISTATUS (Get incubator status) : : : : "ISTATUS" Request incubator status from reader.
1-2-11.
1-2-14. UPLD (Upload the protocol data) : : : : "UPLD" Upload the protocol data : 1 to 66 (1 to 64 for Endpoint protocol, 65&66 for kinetic protocol) Example: EIA.READER UPLD 1 ”ERE” 1-2-15.
1-2-20. SSN (Set Serial Number) : : : "SSN" Set Serial number : Max 5 ASCII characters(“”(0x20) to”~”(0x7e)) Example: eia.reader ssn EP001 ”ERE” 1-2-21. GSN (Get Serial Number) : : : : "GSN" Get Serial number none “ERE” : 5 ASCII characters (The length of serial number is always 5.) Example: eia.
1-3. Absorbance Data 1-3-1. Abs data : [ ] Ref.data present for dual wavelength readings. : “BIO-RAD Model 680 Microplate READER” : ”/” “/” ”:” “:” > ex. “01/01/2001 01:05:02” : “Mes. filter:” “Ref.
1-4. Protocol data All protocol data is ASCII code data. Transmit format: <“,”(0x2c)>< “,”><“,”>…<”,”>”,”(0x2c) delimits each protocol item. Protocol data download or upload orders: Item P.
23 Cutoff negative const value 24 Gray zone 25 Calibrator concentration value Recall setting of Standard 26 curve [3] 27 Number of standard 28 Standard conc.
40 Graph Scale 41 Plate well format data[8][12] Plate well solution number 42 data[8][12] [7]: Difference subtraction 0:Even-Odd (column) 1:Odd-Even(column) 2:Even-Odd(row) 3:Odd-Even(row) [8]: Concentration (“ “(0x20) data delimits print out report types.
1-5. “TSE” and “TSE eq” Assay Parameter Format “TSE” and “TSE eq” parameter is same format. All parameter data is ASCII code data. Transmit format: <“,”(0x2c)><“,”><“,”> …<“,”> “,”(0x2c) delimits each parameters item. “TSE” and “TSE eq” assay data download or upload orders: Item P.
1-6. Upload Raw Data Format 1-6-1. Header Type Format of the Upload Raw Plate Data : < memory #>: (1). End point “End point # " ” : 1 to 10 (End point) ex. “End point #1” (2). Kinetic “Kinetic # " ” 1 or 2 (Kinetic) : "Exist: " : Exist = "1", No exist= "0" ex. “Exist: 1” : ”/” “/” ”:” “:” > ex.
1-6-2-2. Full Data Type Format for Kinetic : < | [] > --- Ref.data present only for dual wavelength reading. : Same as "1-6-1. Header Type Format of the Upload Raw Plate Data" : 0 to 30 "0": It only generates . "1" to "30": this number generates each plate data. (1).
1-7. Download Raw Plate Data Format All protocol data is ASCII code data. Transmit format: <“,”(0x2c)><“,”><“,”>…<“,”> “,”(0x2c) delimits each parameters item. 1-7-1.
Abs. Data Sample of and " 0.101 0.102 0.103 0.104 0.105 0.106 0.107 0.108 0.109 0.110 0.111 0.112"<","> " 0.201 0.202 0.203 0.204 0.205 0.206 0.207 0.208 0.209 0.210 0.211 0.212"<","> " 0.301 0.302 0.303 0.304 0.305 0.306 0.307 0.308 0.309 0.310 0.311 0.312"<","> " 0.401 0.402 0.403 0.404 0.405 0.406 0.407 0.408 0.409 0.410 0.411 0.412"<","> " 0.501 0.502 0.503 0.504 0.505 0.506 0.507 0.508 0.509 0.510 0.511 0.512"<","> " 0.601 0.602 0.603 0.604 0.605 0.606 0.607 0.608 0.609 0.610 0.
(2) Reading number= "1" to "30" 5 Plate reading date 6 Begin of 7 8 9 End of Begin of 10 11 End of ”/”“/””:”“:” : "00" to "99" (2000 to 2099) : "1" to "12" : "1" to "31" : "0" to "23" : "0" to "59" : "0" to "59" "begin" : Abs. Data (See "Abs.
7.3.2 Error Codes Error code 0000 No error 8071 Invalid Command 8072 Parameter out of range Device Not In Remote Mode Device busy Filter wheel 8073 8074 8075 8076 8077 8078 8079 8080 8081 8082 8083 8084 8085 8086 8087 Error The instrument is not in remote mode Light bulb burned out Insufficient light level “reset” command. Incubator error Plate carrier error-1 Plate carrier error-2 Plate carrier error-3 Plate carrier Position Incubator over heat.
4000155 Rev F 64
