Ultramark™ Microplate Reader User Manual 410-0164 Rev B
Ultramark Catalog Number 170-9500 Ultramark User Manual Catalog Number 170-9502 Bio-Rad Technical Services Department Open Monday–Friday, 8:00 a.m. to 4:00 p.m., Pacific Standard Time. Phone: Fax: E-mail: (800) 424-6723, option 2, option 3 (510) 741-6576 (510) 741-5802 LSG.TechServ.US@Bio-Rad.com (U.S.) LSG.TechServ.Intl@Bio-Rad.
Warranty and Regulatory Notices Warranty Statement This warranty may vary outside of the continental United States. Contact your local Bio-Rad office for the exact terms of your warranty. Bio-Rad Laboratories warrants that the Ultramark system will be free from defeats in material and workmanship, and will meet all performance specifications for the period of 1 year from the date of shipment. This warranty covers all parts and labor.
Table of Contents 1. General Information..................................................................................................1 1.1 1.2 Introduction........................................................................................................................ 1 Ultramark Specifications.................................................................................................... 1 2. Equipment Set-up and Installation................................................................
1. General Information 1.1 Introduction The Ultramark microplate reader provides all the flexibility you need for virtually any absorbance assay. You can use it to perform standard colormetric ELISA and kinetic readings for general cell screening with 6, 12, 24, 96, 384, and 1536-well microplate formats. The Ultramark has a 10-magazine filter holder with a working wavelength range of 260–750 nm and an incubation temperature range up to 42 degrees Celsius.
2. Equipment Set-up and Installation 2.1 Unpacking the Ultramark Microplate Reader Before opening the box, check for any outer damage. Document any damages, if present. Place the box upright with the TOP label facing up and open the box. Remove the instrument from the box in this position only. Warning! Grasp the instrument firmly under the right and left sides before attempting to lift or carry it. Check the instrument for damaged or broken parts. Document any damages in detail.
2.4 Location of the Instrument The Ultramark microplate reader is a precise optical measuring instrument. The following conditions must be met to ensure maximum sensitivity: • • • Place the instrument on a flat surface that is free of vibration. Do not place the instrument in direct sunlight. Make sure that the instrument is being operated in a dust-free environment. Warning! Do not operate the instrument with the filter cover off.
3. Using the Ultramark The Ultramark is operated through the Protocol dialog boxes in Microplate Manager. See the Microplate Manager User Guide for instructions on specifying the filter wavelengths (section 2.7) and operating the Ultramark through the Protocol dialogs (Chapter 5). 3.1 Loading a Plate To load a plate into the Ultramark, press the Open/Close button on the front of the instrument or click on the Reader Door: Open button in the Protocol dialog box in Microplate Manager.
4. Changing Filters in the Ultramark This section describes how to change a filter or filters in the Ultramark. To enter filter removing mode, hold down the Open/Close button on the front panel while you turn on the Ultramark. The message window will read “Filter Removing”? Press the Incubator button to continue (the Stop button will exit filter removing mode). The message will read “Remove Magazine.” Remove the two thumbscrews on the right side of the Ultramark and remove the side cover.
Microplate Manager (see the Microplate Manager User Guide, section 2.7). Incorrect wavelength and clip information could result in damage to the Ultramark! Incorrect Correct Make sure that all the filters sit securely and properly in the Magazine clips before reinstalling the Magazine. Reinsert the Filter Magazine into the base so that the pins in the base fit in the holes in the magazine. Insert Screw 2 into the center hole in the Filter Magazine and tighten securely with a coin or screwdriver.
5. Recovering from a Filter Error There are three filter error messages that can appear in the front panel of the Ultramark: • • • Filter Memory Error Filter Set Error Gain Mes-2 Error To correct these errors: 1. Press any button on the front panel of the Ultramark to enter the Filter Set mode. 2. Remove the side panel and the Filter Magazine from the Ultramark (see instructions in previous section). 3. Note the number of the filter that is in the filter arm (i.e.
6. Recovering a Dropped Filter To recover a filter that has dropped inside the instrument, perform the following steps: 1. Turn power off. 2. Loosen thumbscrews on side panel and remove panel.
12 3. Remove thumbscrew holding filter magazine in place. 4. Remove filter magazine. 5. Reach inside instrument, locate dropped filter, and remove. 6. Reinsert filter into clip in magazine, replace the magazine and side panel, and turn the instrument back on. 7. Specify in Microplate Manager that the filter is in the magazine, not the filter arm.
7. Disinfection Procedure All parts of this instrument that can come in contact with patient sera or positive samples must be handled as hazardous items. Bio-Rad Laboratories recommends using gloves when performing maintenance or working with the instrument. It is very important that the instrument undergo thorough disinfection before performing maintenance or before physical removal of the instrument from the laboratory.
8. Error Codes The following error codes appear in the message window of the Ultramark. Error Message Probable Cause Recommended Solution Memory(SRAM) An empty battery. Contact Bio-Rad. ROM replaced. Not a problem. SRAM chip trouble. Contact Bio-Rad. Memory(DRAM) DRAM chip trouble. Contact Bio-Rad. Plate Carrier Dirty guide shaft, or something Confirm that there is nothing blocking blocking the guide shaft. the guide shaft, then clean the shaft and put some grease on it.
Error Message Probable Cause Recommended Solution Xe flash The Xenon Lamp did not flash. If this occurs only a few times a year, it is no problem. Hi-Voltage power supply damaged. Contact Bio-Rad. Xenon Flash Lamp damaged. Contact Bio-Rad. Measuring electronics damaged. Contact Bio-Rad. Analog offset voltage is too high. Contact Bio-Rad. Selected filter is not installed. Install filter. Measuring electronics damaged. Contact Bio-Rad. Xenon Flash Lamp damaged. Contact Bio-Rad.
Error Message Probable Cause Recommended Solution SCSI ini. SCSI chip trouble. Contact Bio-Rad. WCS R/W SCSI SCSI chip trouble. Contact Bio-Rad. DPR R/W SCSI SCSI chip damaged. Contact Bio-Rad. SCSI bus Data communication problem between the Ultramark and your computer. The Ultramark is not the source of the problem. Check your computer. Bus Rst SCSI The termination switch may be Turn the SCSI termination switch on off. (located next to SCSI connector).
9. Product Information 170-9500 Ultramark Microplate Imaging System, 110/230 V, Includes 10 Filters (260, 280, 340, 405, 415, 490, 570, 630, 655, and 750 nm) 170-9501 Feeder/Stacker 170-9518 1536 Well Microplate, 25 170-9519 96 Well UV Transparent Microplate, 25 170-9520 SCSI II Card and Cable for PC 170-9521 MPM 5.
Order Form—Emission Filter Ultramark Microplate Reader Omega series filters (see website www.omegafilters.com for more information) Mechanical Specifications Laminated construction edges sealed. Enclosure: ABS plastic ring. Diameter: 11.9 +0/-0.25mm without ring 14.0 +0/-0.25mm with ring Thickness: 4.9 + 0.1mm without ring 4.9 + 0.1mm with ring Clear aperture: 7.0+0.
Disinfection Certificate This instrument and its inventory have: (1) never been in contact with any dangerous biological material, or (2) been disinfected according to the instructions contained in the operating manual for this instrument.
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