Accessories Instruction Manual

4100172 Rev C 1
1. General Information
1.1 Introduction
The Benchmark Plus microplate reader provides all the flexibility required for absorbance assay.
The Benchmark Plus has the capability to perform standard colormetric ELISA and kinetic
readings for general cell screening with 6, 12, 24, 96, and 384-well microplate formats. The
Benchmark Plus uses a “filter-less” design, with a wavelength range of 340–800 nm. Benchmark
Plus is also provided with an optional incubation feature with temperature range up to 45
degrees Celsius. The optical design provides verifiable results up to 4.0 O.D. The “filter-less”
monochromator design allows the user to set the reading at any wavelength in the 340 – 800 nm
region, as well as do a spectral scan across any wavelength range (within 340 – 800 nm) to find
the absorbance curve.
1.2 Benchmark Plus Specifications
Light Source Xenon Flash Lamp
Spectral Range 340–800 nm
Indication range 0 – 4.000 OD
Resolution 0.001 OD
Monochromator Capacity Any wavelength within the spectral range, with 1 nm step size
Monochromator Bandwidth 5 nm
Wavelength Accuracy, min. ± 2.0 nm
Wavelength repeatability ± 0.2 nm
Shaking Yes
Optional Incubation Up to 45°C , programmable in 0.1° C increments
Power 100–240 V, 60/50 Hz, 2.0 Amps
Dimensions 254 mm (H) x 457 mm (W) x 508 mm (D)
Compatible Plates Any 6- through 384-well microplate with dimensions 138 mm (length) x 98
mm (width). Plate height cannot exceed 15 mm.
Linearity (340 – 800 nm) Less than 2% or 0.01 OD from 0–3.0 O.D.
Accuracy (340 – 800 nm) Less than 1% or 0.01 OD from 0–3.0 O.D.
Reproducibility (340 – 800 nm) Less than 1% or 0.005 OD from 0-3.0 OD
Software Compatibility A System Pentium 166 or higher, 32 Mb RAM, Windows 95 OS or higher
Minimum Kinetic Read Interval 96-well plate: 10 seconds single wavelength
30 seconds dual wavelength
Environmental Operating
Range
5–45 degrees C, max. relative humidity 80% non-condensing