Owner manual

Sample Processing**
6. If using ReadyPrep Mini grinders, centrifuge the tubes
containing resin in a microcentrifuge at 20,000 x g for
20 sec and remove the collected liquid.
7. Weigh out tissue sample (up to 100 mg per ReadyPrep
mini grinder), and add it to the tube containing resin.
8. Add 500 µl of prepared PSB solution to the tube
containing resin.
9. Grind the sample using the supplied matching pestle.
Once larger tissue pieces have been reduced, add an
additional 500 µl of prepared PSB solution. Grinding
times may vary for different tissue samples, but typically
5 min of grinding should be sufficient.
Note: Standardizing the grinding procedure helps ensure
reproducible results (for example, record the length of
grinding time or count the number of pestle turns).
10. Centrifuge the tubes at 20,000 x g for 30 min at 20°C
to pellet the resin and cellular debris.
** For cultured cells, it may not be necessary to use the ReadyPrep mini grinders since
cell lysis will be achieved by vortexing with PSB for 2 min. Cultured cell starting
material should be an approximately 50 µl wet cell pellet. As an example using Jurkat
cells, this will be roughly 15 x 10
6
cells. After steps 1 through 5, begin sample
processing at step 10.
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