Owner manual

Section 4

Instructions for Use

Preparation of Protein Solubilization Buffer (PSB)

Solution

1. Use only freshly rehydrated buffer. Discard any unused

buffer.

2. Allow the PSB dry reagent to warm to room temperature

before opening the bottle. Shake the PSB dry reagent

bottle for 10–15 sec. Weigh an appropriate amount

(each gram of dry reagent will prepare approximately

2 ml buffer solution). Use 1 ml of PSB per 100 mg of

tissue or 50 µl of wet cell pellet (Table 1).

Table 1. Guideline for PSB preparation.

# Samples Volume PSB PSB Dry PSB Diluent Approximate

(100 mg tissue Needed (ml) Reagent (g) (ml) Volume PSB

or 50 µl wet Prepared (ml)

cell pellet)

1 1 1 1.1 2

2 2 2 2.2 4

3 3 2 2.2 4

4 4 3 3.3 6

5 5 3 3.3 6

3. For each gram of dry reagent, add 1.1 ml of PSB

diluent.

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