Owner manual

Section 1

Introduction

MicroRotofor lysis kits provide convenient, effective methods

for the preparation of protein samples for fractionation with

the MicroRotofor cell. The MicroRotofor lysis kit (mammal)

is designed for use with mammlian tissues and cell cultures,

and employs tissue maceration and/or solubilization into a

chaotropic extraction buffer (Vuillard et al. 1995). For added

convenience, the extraction buffer is also used as the

sample buffer for isoelectric focusing (IEF) with the

MicroRotofor cell or with IPG strips. Though the kit is

intended for use with mammalian tissues, it may be applied

to other animal tissues and to prepare samples for purposes

other than IEF with the MicroRotofor cell, such as IEF with

IPG strips, 2-D analysis, or SDS-PAGE.

Section 2

Kit Specifications

Each MicroRotofor lysis kit (mammal) provides sufficient

reagent to perform at least 15 extractions (from 100 mg

tissue samples) and to prepare samples for 15 MicroRotofor

runs. More than 15 extractions will be possible if samples

are smaller than 100 mg or if the sample is applied onto

IPG strips (and not prefractionated with the MicroRotofor

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