Owner manual

Section 5
Appendix
Preparation for SDS-PAGE
CHAPS, a component of the PSB diluent, may interfere
with SDS-PAGE. Remove CHAPS from the extracts (for
example, with the ReadyPrep 2-D cleanup kit), or dilute the
extracts 1:1 with 1x Laemmli buffer prior to SDS-PAGE.
Preparation for IEF on an IPG Strip
Following step 13, the sample extract can be loaded
directly onto an IPG strip after appropriate dilution. See
Table 3 for recommendations on how much protein sample
to load onto an IPG strip. Dilution of the sample can be
done using protein solubilization buffer (PSB) as a
rehydration/sample buffer. However, some critical
components must be added to the PSB solution to make it
IEF-compatible (Table 2).
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