Manual

Section 4

Instructions for Use

Preparation of Protein Solubilization Buffer (PSB)

Solution

1. Use only freshly rehydrated buffer. Discard any unused

buffer.

2. Allow the PSB dry reagent to warm to room

temperature before opening the bottle. Shake the PSB

dry reagent bottle for 10–15 sec. Weigh an appropriate

amount (each gram of dry reagent will prepare

approximately 2 ml buffer solution). Use 1 ml of PSB

per 50 µl of wet cell pellet (Table 1).

Table 1. Guideline for PSB preparation.

# Samples Volume PSB PSB Dry PSB Diluent Approximate

(50 µl wet cell Needed (ml) Reagent (g) (ml) Volume PSB

pellet) Prepared (ml)

1 1 1 1.1 2

2 2 2 2.2 4

3 3 2 2.2 4

4 4 3 3.3 6

5 5 3 3.3 6

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