Manual

Items Required But Not Provided
• 1.5 ml microcentrifuge tubes
• Microcentrifuge capable of spinning at 20,000 x g
• DTT reducing agent (catalog #161-0611) or TBP reducing
agent (catalog #163-2101)
• Carrier ampholytes
• RC DC
™
protein assay (catalog #500-0121 or 500-0122)
• ReadyPrep
™
proteomic grade water (catalog #163-2091)
or other ultrapure water
• 37°C heat block or water bath
• Sonicator with probe
Items Recommended But Not Required
• Protease inhibitor (for example, Sigma catalog #P8465)
• ReadyPrep reduction-alkylation kit (catalog #163-2090)
• ReadyPrep 2-D cleanup kit (catalog #163-2130)
Section 3
Storage Conditions
Shipped at ambient temperature. Store kit components as
individually marked. Note: Lysozyme is shipped at room
temperature but should be stored at –20°C upon receipt.
This kit has a warranty period of 1 year from shipment
date, assuming all components are stored as indicated on
each label.
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