Manual

This kit can be used with both gram negative and gram
positive bacteria. Each MicroRotofor run using 2.5 mg total
protein yields ten 150–250 µl fractions, and the protein
distribution among the fractions will vary depending on the
sample. For example, using E. coli extracts and ampholytes
spanning the pH range 3–10, fractions 3–6 typically contain
the highest amounts of protein.
Certificates of analysis and MSDS forms are available upon
request.
Items Supplied With Kit
Protein solubilization buffer (PSB) (contains urea, 25 g
thiourea, NDSB 201, and Tris)
PSB diluent (contains CHAPS and Tris) 30 ml
Bacteria suspension buffer (contains sodium 25 ml
phosphate, sodium chloride, potassium chloride,
potassium phosphate)
Lysozyme enzyme, 1,500 units/µl, prepared from 1.0 ml
chicken egg, MW = 14.3 kD, pI = 9.32
(Swiss-Prot/TrEMBL acession number
LYSC-CHICK P00698)
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