Manual

• DTT reducing agent (catalog #161-0611) or TBP reducing
agent (catalog #163-2101)
• Carrier ampholytes
• RC DC
™
protein assay (catalog #500-0121 or 500-0122)
• Glycerol
• ReadyPrep proteomic grade water (catalog #163-2091)
or other ultrapure water
• Liquid nitrogen
• Mortar and pestle
Items Recommended But Not Required
• Protease inhibitor (for example, Sigma catalog #P9599)
• ReadyPrep reduction-alkylation kit (catalog #163-2090)
• Bio-Rad spin filters (catalog #700-7254) or equivalent
filtration device
Section 3
Storage Conditions
Shipped at ambient temperature. Store kit components as
individually marked. Wash reagent 2 (component of the
ReadyPrep 2-D cleanup kit) must be stored at –20°C for
at least 1 hr prior to use. For convenience, store wash
reagent 2 at –20°C. This kit has a warranty period of 1 year
from shipment date, assuming all components are stored
as indicated on each label.
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