Owner's manual

Student Transformation Protocol

Listed are materials and reagents required at the workstations prior to

beginning the transformation activity.

Instructor’s (Common) Workstation Quantity (✔)

Water bath, heating block, or incubator (37°C) 1 ❒

Microcentrifuge (refrigerated, if available) 1 ❒

Each student team will require the following items to transform bacteria

with one PCR product.

Materials Required at Each Student Station Quantity/Team (✔)

Ligation reaction from ligation laboratory (on ice) 5 µl ❒

(Optional) Control plasmid 50–200 ng/µl (on ice) 1 µl ❒

1.5 ml microcentrifuge tubes 4 ❒

15 ml culture tube containing 1.5 ml of 1 ❒

C-growth medium

Transformation reagent A (on ice) 250 µl

❒

Transformation reagent B (on ice) 250 µl ❒

Sterile inoculating loops 2 ❒

Fresh LB Amp IPTG agar plate prewarmed to 37°C 1–2 ❒

Fresh starter culture plate of

E. coli

HB101 1 ❒

200 µl adjustable-volume micropipet and tips 1 ❒

1,000 µl adjustable-volume micropipet and tips 1 ❒

Marking pens 1 ❒

Parafilm sealing film 1 strip ❒

(Optional) Vortex mixer 1 ❒

(Optional) Vacuum source 1 ❒