Accessories User Manual

Econo-Column Flow Adaptor
Reagent 20°C 50°C 20°C 50°C
Citric acid E E E E
Cresol G F G F
Cyclohexane F N F N
Cyclohexanone F N F N
Dimethyl formamide N N N N
Dimethylsulfoxide - - - -
Ethyl acetate N N N N
Ethyl alcohol E G E G
Ethyl benzene - - - -
Ethyl chloride, liquid F N F N
Formaldehyde, 40% E G E G
Formic acid, 98–100% E G E G
Glycerine E E E E
Hydrochloric acid, 35% E G E G
Hydrofluoric acid, 48% E E E E
Hydrogen peroxide, 90% E - E -
Isobutyl alcohol E E E E
Isopropyl alcohol E E E E
Lactic acid, 85% G F G F
Methylene chloride N N N N
Nitric acid, 10% E E E E
Nitric acid, 50% F N F N
Nitrobenzene N N N N
Perchloric acid G N G N
Phenol, liquid N N N N
Phosphoric acid, 5% E E E E
Phosphoric acid, 85% E G E G
Propionic acid E G E G
Sodium acetate, saturated E E E E
Sodium hydroxide, 1% E E E E
Sodium hydroxide, 50% to saturated E G E G
Sodium hypochlorite G F G F
Sulfuric acid, 6% E E E E
Sulfuric acid, 60% E G E G
Sulfuric acid, 98% F N F N
Toluene G F G F
Trichloroacetic acid F N F N
Trichloroethane N N N N
Trichloroethylene N N N N
Tris buffer, solution E G E G
Urea E E E E
Xylene F N F N
Reprinted by permission of Nalge Company
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Pressure Stability
Econo-Column chromatography columns are designed for low-pressure applica-
tions, i.e., less than 1.0 atm. Use caution when operating the column with a
pump. A disruption in flow may cause a rapid rise in backpressure. If this occurs,
immediately shut off the pump and check the gel bed for compression.
Temperature Stability
Econo-Column chromatography columns and flow adaptors can be operated at
temperatures up to 50°C. For applications requiring temperature control, jacket-
ed columns are available in various sizes and formats. Refer to the Bio-Rad Life
Sciences Research Products catalog for more information.
Sterilization
Econo-Column chromatography columns and accessories can be sterilized with
2 N NaOH or 100% ethanol. The columns can also be autoclaved, wet or dry, at
a maximum of 15 min at 121°C.
Column Cleaning
Econo-Column chromatography columns can be cleaned with 70% ethanol. The
bed support pore size is 20 µm and may require intermittent cleaning. To clean
the bed support, backwash with 1 N NaOH.
Accessories
A full range of Econo-Column accessories are also available. For more detailed
ordering information, refer to the Bio-Rad Life Science Research Products cata-
log.
Flow Adaptors
Flow adaptors improve resolution and reduce mechanical disruption by deliver-
ing sample and buffer directly to the top of the gel bed.
Catalog# Description
738-0014 Flow Adaptor, 1 cm ID, 7 cm long
738-0015 Flow Adaptor, 1 cm ID, 14 cm long
738-0016 Flow Adaptor, 1.5 cm ID, 14 cm long
738-0017 Flow Adaptor, 2.5 cm ID, 14 cm long
738-0018 Flow Adaptor, 5.0 cm ID, 14 cm long
738-0019 Econo-Pac
®
Flow Adaptor, 1.5 cm ID, 7 cm long
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