Software Updates User guide

CFX96 Touch, CFX96 Touch Deep Well, CFX Connect, and CFX384 Touch Systems Instruction Manual

3 Performing Runs

Read this chapter for information about performing runs using CFX Manager™ software:

• Run Setup window (page 27)

• PrimePCR™ runs (page 28)

•Protocol tab (page29)

• Plate tab (page 29)

• Start Run tab (page 30)

• Run Details window (page 31)

• Instrument Summary window (page 34)

Run Setup Window

The Run Setup window provides quick access to the files and settings needed to set up and

start a run. To open the Run Setup window, perform one of these options:

• Click the User-defined or the PrimePCR button in the Run Setup tab of the Startup

Wizard (page 17)

• Click the User-defined Run Setup or PrimePCR Run Setup button in the main

software toolbar (page 16)

•Select File > New > User-defined Run or PrimePCR Run in the main software

menu bar (page 14)

The Run Setup window includes three tabs:

•Protocol. Click the Protocol tab to select an existing protocol to run or edit, or to create

a new protocol in the Protocol Editor window (page 37)

•Plate. Click the Plate tab to select an existing plate to run or edit, or to create a new

plate in the Plate Editor window (page 45)

• Start Run. Click the Start Run tab (page 30) to check the run settings, select one or

more instrument blocks, and begin the run

NOTE: If the protocol currently selected in the Protocol tab does not include a step

with a plate read for real-time PCR analysis, then the Plate tab is hidden. To view

the Plate tab, add a “Plate Read” (page 39) in at least one step in the protocol.

NOTE: Start a new run from a previous run by selecting File > Repeat a Run in the

main software menu bar or Repeat Run in the Startup Wizard. Select the data file

(.pcrd) for the run you want to repeat.