Software Updates User guide

CFX96 Touch, CFX96 Touch Deep Well, CFX Connect, and CFX384 Touch Systems Instruction Manual
127
Where:
• E = Efficiency of primer and probe set. This efficiency is calculated with the formula
(% Efficiency * 0.01) + 1, where 100% efficiency = 2
•C
q (control)
= Average C
q
for the control sample
•C
q (sample)
= Average C
q
for any samples with a GOI
• GOI = Gene of interest (one target)
Standard Deviation of Relative Quantity
The standard deviation of the relative quantity is calculated with the following formula:
Where:
• SD Relative Quantity = standard deviation of the relative quantity
•SD C
q
sample = Standard deviation of the C
q
for the sample (GOI)
• Relative Quantity = Relative quantity of the sample
• E = Efficiency of primer and probe set. This efficiency is calculated with the formula
(% Efficiency * 0.01) + 1, where 100% efficiency = 2
• GOI = Gene of interest (one target)
Efficiency Corrected Cq (Cq
E
)
The efficiency corrected Cq is calculated by the following formula:
Where:
• E = Efficiency
Mean Efficiency Corrected Cq (MCq
E
)
The mean efficiency corrected Cq is calculated with the following formula:
Where:
•Cq
E
= Efficiency corrected Cq
• n = Number of replicates
SD Relative Quantity SD Cq
GOI
Relative Quantity
Sample X
Ln (E
GOI
××)=
Cq
E
Cq (log(E)/log(2))×=
MCq
E
Cq
E (Rep 1)
Cq
E (Rep 2)
… Cq
E (Rep n)
+++
n
----------------------------------------------------------------------------------------------------------
=