Software Updates User guide

Gene Expression Analysis
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The Study Setup tab lists the data files in the Gene Study, as described in Table 43.
Study Analysis Tab
The Study Analysis tab shows the data from all runs that are added to the Gene Study. The
gene expression data analysis options available are the same as those for a single data file
with the following exceptions:
• For bar charts, inter-run calibration values, if calculated, will be displayed by clicking
the Inter-run Calibration button
• For heat maps, if the same targets are in the same location on multiple plates, but
with different samples, use the drop-down menu to select a particular plate for
analysis
Gene Study Report Window
Open the Gene Study Report window to arrange the Gene Study data into a report. To create a
gene study report, follow these steps:
1. Adjust the Gene Study report data and charts as needed before creating a report.
2. Select Tools > Reports to open the Gene Study report window.
3. Click the checkboxes in the report options list to select and remove options to choose
the data to display. Select the options shown in Table 44.
Table 43. Study Setup tab in the Gene Study window.
Column Title Description
File Name Name of the run data file (.pcrd extension)
File Folder Directory that stores the data file for each run in the Gene
Study
Date Created Date the run data were collected
Well Group Name Name of the well group that was selected when the file was
added to the Gene Study
TIP: In order to analyze one well group in the Gene Study,
that well group must be selected in the Data Analysis
window before importing the data file into the Gene Study
Step Protocol step that included the plate read to collect real-
time PCR data
Run Type Indicates user-defined or PrimePCR run
Protocol Edited If checked, indicates that the prorotocl used for a
PrimePCR run has been edited
Plate View Open a plate map of the plate with the data in each of the
runs included in the Gene Study
Table 44. Categories for a Gene Study report.
Category Option Description
Header Title, subtitle, and logo for the report