Software Updates User guide

CFX96 Touch, CFX96 Touch Deep Well, CFX Connect, and CFX384 Touch Systems Instruction Manual
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Data Spreadsheet
The spreadsheet lists the target, sample, regulation, P value, and whether the P value exceeds
the threshold, and it indicates up- or downregulation compared to the threshold setting. Click
the checkbox adjacent to a target to include or exclude it from the plot. Select chart options by
right-clicking.
Heat Map
The heat map presents a visual depiction of the regulation of targets for an experimental
sample compared to a control sample based on relative normalized expression and its location
on a plate.
A legend below the heat map shows the range of normalized expression, which corresponds
to the following:
Upregulation (red). Higher expression
Downregulation (green). Lower expression
No change (black)
The lighter the shade of color, the greater the relative normalized expression difference. If no
normalized expression value can be calclulated, the square will be black with a white X.
Settings
The following can be set:
Control Sample
Experimental Sample
Size. Image size can be adjusted using the slider to alter the degree of chart
magnification for easier visualization
Split Out Replicates. Show values for the individual replicates
Right-Click Menu Options
Right-click on the heat map to select the items shown in Table 40.
Data Spreadsheet
The spreadsheet lists the target, sample, and regulation. Select chart options by right-clicking.
Results
The Results spreadsheet summarizes the data from all the charts. The contents of the Results
spreadsheet are listed in Table 42.