Software Updates Instruction Manual

Index
134
Opening
Lid, 26
Melt Curve tab, 74
Protocol AutoWriter, 37
Protocol Editor, 31
Operation requirements, 3
P
Pause
Button, 27
Run, 27
Plastic Consumables, 8
Plate
Contents of wells, 45
Melt Curve Data, 76
Size, in Plate Editor, 40
Spreadsheet, 51, 76
Type, 41
Well contents, 45
Plate Editor
Clear Replicate #, 47
Clear Wells button, 47
Clearing wells, 63
Concentration, 46
Dilution Series, 47
Experiment Settings, 47
Export plate spreadsheet, 51
Import plate spreadsheet, 51
Magnification, 41
Menu bar, 40
Plate size, 40
Plate Type, 41
Replicate #, 46
Replicate Group, 46
Replicate series, 46
Sample Name, 46
Sample Type, 45
Scientific notation, 41
Selecting fluorophores, 44
Spreadsheet, 51
Target Name, 45
Toolbar, 41
Units, 41
Well Groups Manager, 41
Well Notes, 47
Power
Input, 2
Switch, 2
Protocol
Block mode, 37
Calculated mode, 37
End point, 23
Express load, 23, 24
Steps, temperature, 31
Protocol AutoWriter, 37
Amplicon length, 37, 38
Annealing temperature, 37
Button, 37
Creating a protocol, 37
Protocol Editor
Buttons, 33
Delete step, 36
Inserting GOTO, 34
Inserting gradient, 34
Inserting melt curve, 35
Menu bar, 32
Window, 31
Q
QC Tab, 82
Quantitation
Description, 56
Tab, 56
Tab layout, 56, 68
R
Ramp rate, 36
Reaction efficiency (E)
Formula, 70
Targets, 70
Real-Time Status, 27
Regression Mode, 58
Relative quantity
Control sample selected, 106
Described, 92
Formula, 106
GOI, 106
Normalization factor, 107, 108
Normalized expression, 109
Standard deviation formula, 107
Replace Plate File, 28
Replicate #, 46
Replicate Group, 46
Replicate Series, 46
Report
Data file, 84
Gene Study, 104
Requirements
Operation, 3
Resume button, 27
Resuming a run, 27
RFU