Software Updates Instruction Manual

MiniOpticon Instruction Manual
133
Right-click for graph, 94
Scaling options, 93
Spreadsheet, 94
Gene Study, 101
Inter-run calibration, 101
Preparing data, 103
Report, 104
Show Details, 104
Study Analysis tab, 102, 104
Study Setup tab, 103
GOI
Normalization factor, 107, 108
Normalized expression, 108, 109, 110
Relative quantity, 106, 107
SD Normalized expression, 110
GOTO
Adding repeats, 26
Inserting, 34
Gradient
Inserting, 34
Step, 34
Gradient Calculator, 32
H
Heated plate, 2
I
Import Template, 51
Increment, 36
Inner lid, 2
Instruments
Safety, iii
Warning labels, iii
Inter-run calibration, 101
L
Layout
Quantitation tab, 56, 68
LED on lid, 2
Library, 45
Lid
Green LED, 2
Opening, 26
M
Magnification in Plate Editor, 41
Master Mix Calculator, 15
Melt Curve
Adjusting data, 75
Data analysis, 74
Inserting, 35
Opening tab, 74
RFU data, 74
Melt Curve Data, 76
Amplification Data (RFU), 77
Plate, 76
Tab, 75
Melt Peaks
Spreadsheet, 76
Melt Peaks Spreadsheet, 76
Menu bar
Data analysis, 55
Main software, 10
Plate Editor, 40
Protocol Editor, 32
Mode
Baseline Subtracted, 59
Baseline Subtracted Curve Fit, 59
Not Baseline Subtracted, 59
Temperature control, 36
Multiple instruments
Viewing, 14
M-value, 93
N
Normalization factor
Relative quantity, 107, 108
Normalized expression
Formula, 108
Formula for GOI, 111
Scaled to highest, 109
Scaled to lowest, 109, 110
Standard deviation (SD), 110
Standard deviation formula, 109
Normalized GOI formula, 111
Normalizing
Gene expression, 91
Not Baseline Subtracted mode, 59
Notes
Well, 45
O
Open Lid button, 26