Software Updates Instruction Manual
Index
132
Sample volume, 23, 33
Thresholds, 59
Chart
Data analysis, 63
Cleanup events, 19
Clear Replicate #, 47
Clear Wells button, 47
Clearing wells in Plate Editor, 63
Close button for lid, 2
Close Lid button, 26
Closing the lid, 26
Coefficient of Variation, 93
Color, 44, 49, 97
Concentration, 45, 46
Control
Relative quantity with, 106
Creating
Data report, 85
Protocol, 37
Well groups, 50
Custom Data View, 81
Custom Export, 65
Cycles to Analyze, 60, 61
D
-d(RFU)/dT
Spreadsheet, 77
Data analysis
About, 53
Adjusting, 53
Charts, adjusting, 63
Clearing wells, 63
Data analysis, 79
End Point, 77
Formula, 106
Gene Expression, 89
Melt Curve, 74
Menu bar, 55
Plate content, 53
Toolbar, 54
Well contents, 53
Well groups, 50
Well selector, 61
Data file
Report, 84
Data report
Creating, 85
Delete Step button, 36
Deleting a step, 36
Detected Instruments, 9
Dilution Series, 47
E
Email Setup in CFX Manager, 115
Email Tab, 115
End Point
Adjusting data, 78
Changing sample volume in protocol, 23
RFU data analysis, 77
Spreadsheet, 79
Tab, 77
End Point Only Run protocol, 23
Enzyme type, 38
Experiment Settings, 47
Auto Efficiency, 49, 96
Button, 48
Color, 49, 97
Show Analysis Settings button, 49, 96
Show Graph, 49, 97
Targets tab, 48, 95
Export All Data Sheets to Excel, 55, 65
Export RDML Files, 65
Export Template, 51
Export to LIMS Folder, 66
Express Load
Protocol, 23, 24
Extend, 36
F
Flash Block Indicator button, 27
Fluorophore Analysis Mode, 60
Fluorophores, 45
Formula, 107
Data analysis, 106
E (see reaction efficiency), 70
Normalized expression, 108, 109, 110
Normalized expression, scaled, 109
Reaction Efficiency (E), 70
Relative quantity, 106
SD normalized expression, 110
Standard error, 111
Standard error normalized expression, 111
Front view, 2
G
Gene expression
Adjusting data, 92
Data analysis, 89
Graph data, 92
Normalizing data, 91
Relative quantity, 92










