Software Updates Instruction Manual

MiniOpticon Instruction Manual

111

P-Value

The p-value is a measure of the statistical significance of an experimental sample data point

compared to a control.

NOTE: At least two replicates are required for both control and experimental

samples for a p-value to be determined. The more replicates that are present, the

greater the accuracy.

The p-value is calculated as follows:

Where:

•

Where:

•  = gamma function

• t = t-statistic

Where:

• Mean = Arithmetic mean

• NE = Normalized expression

• Count(x) = Size of list x

• SD = Sample standard deviation

Corrected Values Formulas

A difference between corrected values and noncorrected values is seen only if a standard

curve is created as part of the real-time PCR run. The software uses three equations in

determining the error propagation:

• Standard Error

• Standard Error for Normalized Expression

• Standard Error for the Normalized Gene of Interest (target)

The formula for standard error is shown here:

v Count (NE

sample (Experimental)

 Count (NE

sample (Control)

 2–+=

Standard Error

--------=