Software Updates Instruction Manual

Gene Expression Analysis
94
Right-Click Menu Options
Right-click on the Gene Expression graph to select the items shown in Table 35.
Data Spreadsheet
Table 36 describes the information shown in the Gene Expression spreadsheet.
Table 35. Right-click menu items
Item Function
Copy Copy the chart to a clipboard
Save as Image Save the graph in the chart view as an image file. The default
image type is PNG. The other selections for image file types
include GIF, JPG, TIF, and BMP
Page Setup... Select a page setup for printing
Print... Print the chart view
Show Point Values Display the relative quantity of each point on the graph when
you place the cursor over that point
Set Scale to Default Set the chart view back to the default settings after magnifying
it
Chart Options... Open the Chart Options window to adjust the graph
Sort Sort the order that samples or targets appear on the chart X-
axis
User Corrected Std Devs Calculate the error bars using the corrected standard deviation
formula
Use Solid Bar Colors Display solid bars in the graph
X-axis labels Choose to display x-axis labels horizontally or angled
Table 36. Description of information in the spreadsheet on the Gene Expression tab.
Information Description
Target Target Name (amplified gene) selected in the Experiment
Settings window
Sample Sample Name selected in the Experiment Settings window
Ctrl Control sample, when the Sample Name is selected as a
control in the Experiment Settings window
Expression Normalized Gene Expression (C
q
) or Relative quantity (C
q
)
depending on the selected mode
Expression SEM (or SD) Standard Error of the Mean or Standard Deviation, depending
on the selected option
Corrected Expression SEM
(or SD)
Corrected value calculation for Standard Error of the Mean
(SEM) or Standard Deviation (SD) of the relative expression,
depending on the selected option
Mean C
q
Mean of the quantification cycle
C
q
SEM (or SD) Standard Error of the Mean or Standard Deviation of the
quantification cycle, depending on the selected option