User guide

QX200 Droplet Reader and QuantaSoft Software Instruction Manual QX200 Droplet Reader and QuantaSoft Software Instruction Manual | 3
Chapter 1 QX200 Droplet Digital PCR System
Table 1.2. Additional materials required.
Component
R
ecommended
C
atalog # and Supplier
Q
X200 droplet generator
Reagents for probe detection
PCR supermix
d
dPCR supermix for probes
1
86-3010, 186-3026, 186-3027,
1
86-3028
D
roplet PCR supermix
1
86-3023, 186-3024, 186-3025
O
ne-Step RT-ddPCR supermix for probes
1
86-3021, 186-3022
Droplet generator oil Droplet generator oil for probes 186-3030, 186-3005
Control
d
dPCR buffer control kit for probes
1
86-3052
Reagents for EvaGreen detection
PCR supermix
Q
X200
™
ddPCR
™
EvaGreen
®
supermix 186-4033, 186-4034, 186-4035,
186-4036
Droplet generator oil
Q
X200 droplet generator oil for EvaGreen dye
1
86-4005, 186-4006
Control QX200 buffer control kit for EvaGreen dye 186-4052
Consumables and other materials
Pipets 20 μl pipet for sample loading Rainin L-20
5
0 μl pipet for droplet transfer
R
ainin L-50, L8-50
8
-channel, 200 μl pipet for oil
R
ainin L8-200
Pipet tips Filtered Rainin GP-L10F, GP-L200F
96-well PCR plates
t
win.tec semi-skirted 96-well plate
E
ppendorf 951020362
Reagent trough Any
F
oil plate seals
P
ierceable foil plate seals
1
81-4040
Plate sealer PX1
™
PCR plate sealer 181-4000
8-cap strips
A
ny
QX200 droplet reader
Droplet reader oil
D
roplet reader oil
1
86-3031, 186-3004
Droplet reader waste bottle Empty waste bottle N/A