User guide

QX200 Droplet Reader and QuantaSoft Software Instruction Manual2 |
Chapter 1 QX200 Droplet Digital PCR System
1.2 System Components
The system consists of two instruments and associated software, consumables, and reagents (Table 1.1):

QX200 droplet generator — utilizes proprietary reagents and microfluidics to partition samples into 20,000
nanoliter-sized droplets

QX200 droplet reader — following PCR amplification of the nucleic acid target in the droplets, this
instrument analyzes each droplet individually using a two-color detection system (set to detect FAM and
either VIC, HEX, or EvaGreen); PCR-positive and PCR-negative droplets are counted to provide absolute
quantification of target DNA in digital form using QuantaSoft
™
software
Additional materials required for performing ddPCR are listed in Table 1.2.
Table 1.1. QX200 Droplet Digital PCR system components. Items shipped with the QX200 ddPCR system
(catalog #186-4001). Catalog # refers to replacement items (quantities may be different).
Component Description Catalog #
Q
X200 droplet generator
QX200 droplet generator
I
nstrument used for droplet generation
1
86-4002
DG8
™
droplet generator Microfluidic cartridge used to mix sample and oil to 186-4008
cartridges and gaskets (24) generate droplets; gaskets seal the cartridge to
prevent evaporation and apply pressure required
for droplet formation
Droplet generator cartridge
P
ositions and holds the droplet generator cartridge in
1
86-3051
h
older
t
he instrument for droplet generation
Power cord Connects QX200 droplet generator to power source Call technical support
QX200 droplet reader
QX200 droplet reader
I
nstrument used for droplet reading, data collection
1
86-4003
Laptop PC and QuantaSoft software
C
onnects to QX200 droplet reader for data collection
1
86-3007
a
nd analysis
Droplet reader plate holders (2)
P
osition 96-well PCR plate in the droplet reader
5
10-10608
USB 2.0 cable
C
onnects QX200 droplet reader to PC
C
all technical support
Power cord
C
onnects QX200 droplet reader to power source
C
all technical support
QX200 Droplet Digital PCR system. Left, QX200 droplet reader. Right, QX200 droplet generator.