Software Manual

Evaluating Fractions
User Guide | 245
Calculating Protein Concentration for Fractions
You can calculate and view the protein concentration for individual and pooled
fractions after you perform peak integration. Peak integration is performed using
default settings. If necessary, you can adjust the baseline by changing the Best Fit
or Offset parameters and reintegrating. The baseline is used to calculate the area
under the curve of the UV trace in each fraction. See Baseline Parameters on
page 228 for more information.
For pooled fractions, the protein concentration is calculated by a weighted average
of the fractions included in the pool.
To calculate protein concentration for individual or pooled fractions
 Click Peak Integration on the Run tab toolbar.
After you perform peak integration, the following columns appear in the Fractions
table:
 Area
 Relative Area
 Extinction Coefficient
 Concentration
 Amount
The extinction coefficient and concentration fields are automatically populated
when the extinction coef
ficient is entered in the Peaks table. If a fraction spans
multiple peaks that have different extinction coefficient values, these values are not
imported and the extinction coefficient field in the Fractions table displays the word
Multiple. In this case, you can manually enter the extinction coefficient field in the
Fractions table.
You can manually change the extinction coefficient of a fraction in the Fractions
table. Doing so will not change the coef
ficient of the peak in the Peaks table.