Software Manual

Peak Integration
User Guide | 235
 Molecular mass (kDa) — the molecular mass of the protein. By default
this field is empty. Molecular mass values that you enter in the Peaks table
are automatically populated to the Fractions table for relevant fractions.
Molecular mass values that you enter in the Fractions table are not
automatically populated to the Peaks table.
 Molarity (M) — the calculated molar concentration of the protein for the
specified peak. This value is computed from the calculated concentration
and molecular mass.
 280/260 (or 280/255) — the ratio of absorbance at 280 nm and 260 nm (or
255 nm) used to determine purity of protein for each peak in the 280 trace.
The ratio appears in the 280 nm trace section of the table. The ratio is
calculated using baseline-subtracted values of the UV trace at the
indicated retention time or volume of the 280 nm peak.
Note: This column appears in the Peaks table after peak integration is
performed when both the 280 nm and 260 nm (or 255 nm) traces ar
e
detected. Depending on which trace is present, the column name can be
either 280/260 or 280/255.
Table Display Order and Column Selection
You can change the order of the table columns. Column selection and order settings
are specific to the user and apply to subsequent peak integration results tables.
To change column display order
 Drag columns to new locations in the Peaks table.
Showing or Hiding Columns
As in the Runs/Traces table, you can show or hide columns in the Peaks table by
choosing Show Column Chooser in the context menu.
Note: You can show or hide columns in the table without affecting the data the
columns contain.
To hide columns in the displayed Peaks table
 Click the column heading to hide and drag it out of the table.