Software Manual

Creating a Method from Standard Phases
User Guide | 169
Serpentine is selected by default. Fractions can also be collected by rows or
columns for microplates. Possible choices appear for the rack you select.
If you have outlet valves in the fluidic scheme, choose a starting valve and port
and modify the fraction size.
8. Under Flow, specify the default flow rate for the method. You can change the
default flow rate within individual phases if needed.
Select the Control the flow to avoid overpressure checkbox if you want the flow
rate to decr
ease and the method to continue running in the event that the
system r
eaches high pressure limits. If you do not select this checkbox the
system pumps will stop, pausing the method.
If you have an air sensor installed and you want to monitor and stop the system
pumps if air is detected in the lines, select the Detect end of buf
fer with Air
Sensor checkbox.
Note: If the method pauses due to an end-of-buf
fer signal, the fluidic lines
must be primed befor
e r
esuming the method. Because the system pump’s
Purge button is inactive in Method mode, you must reprime the pumps
manually. See Priming and Purging the System on page 94 for information