Software Manual

Phase Controls and Parameters
User Guide | 145
System CIP (Clean in Place) — Storage Parameters
Note: System CIP must be run as the only type of phase in the method. You
can add several System CIP phases to the method if multiple cleaning solutions
ar
e required. Bio-Rad recommends that you save System CIP as a separate
method.
If the system has been exposed to hazardous biological material, run System CIP
and Column CIP to flush the entir
e system tubing with sanitizing solution (for
example, NaOH) followed by neutral buffer and finally distilled water before service
or maintenance.
This phase cleans the system after purification runs by rinsing the system with
cleaning solution. Perform System CIP when r
equired; for example, between runs
where different samples and buffers are used or before storing the system. This
helps prevent cross-contamination between runs and prevents bacterial growth in
the instrument during storage.
Depending on the modules in the fluidic scheme, choose the scope of cleaning by
selecting checkboxes for the dif
ferent valves, ports, pumps, column positions,
modules, and loops to clean and fill with cleaning solution. The System CIP method
includes two System CIP phases to facilitate the use of two different solutions; for
example, NaOH, buffer solution, or distilled water. Select values for flow rate,
volume per position, and incubation time.
Note: Each phase uses one cleaning solution. All inlet lines selected in one
phase should be immersed in the same cleaning solution.
Special Considerations
When the fluidic scheme includes a buffer blending valve, NGC systems perform
System CIP differently depending on whether the buffer selection is manually
prepared via the buffer blending valve, blended through the buffer blending valve, or
manually prepared through buffer inlet valves. Note the following special
considerations regarding System CIP with a buffer blending valve in the fluidic
scheme.