Software Manual

Phase Controls and Parameters
User Guide | 143
Injecting Sample with an Autosampler
If your fluidic scheme includes an autosampler and you choose to use manually
prepared buffer via inlet valves in Method Settings, you have the option to use the
same inlets as defined in Method Settings or to select specific buffers from each
port.
Column Wash Parameters
This phase washes the column of impurities and unbound sample. The wash can be
programmed to occur for a defined time or volume or to continue until a certain UV
or conductivity value has been attained. You can also activate fraction collection
during this phase.