Software Manual

Phase Controls and Parameters
User Guide | 137
In gradient segments, lower numbers yield increasing gradients. Higher
numbers yield negative gradients.
 Load/Inject Sample — controls sample loop loading, sample injection,
and loop washing.
 Fraction Collection — turns on fraction collection for the phase, sets the
fraction collection scheme, enables you to select the primary collector
(BioFrac fraction collector or outlet valve), sets the start tube or port, and
sets the fraction size.
 Change Valve — changes the selected valve to the selected position.
 Lamp Control — sets the wavelength and turns the lamp on/off for the
detector selected in the fluidic scheme.
 Zero Baseline — sets zero as the baseline for the UV detector selected in
the fluidic scheme.
 Hold Until — keeps the phase at the current composition until a button is
pressed, timeout occurs, or a threshold value is reached.
 Pause — pauses the method until a button has been pressed or a timeout
time has been reached.
 System Wash — sequentially switches through pumps, valves, and
sample loops and flows a defined volume of cleaning solution through
each position at a defined flow rate.
Note: This step is not supported when either Gradient Segments or Load
Inject Sample steps ar
e included in the method.