Software Manual

5 | Method Editor
134 | NGC Chromatography Systems and ChromLab Software
 Buffer Blending — buffer acid, buffer base, water, and salt are mixed
together to form a buffer of a specified pH, buffer concentration, and salt
concentration. A buffer system (recipe) is selected for use, after which the
acid, base, water, and salt solutions are prepared and fed to the pumps
through the buffer blending valve ports, Q1–Q4. Gradients are formed by
the blending valve rather than the pumps. Because of this, both pumps
can run at their full capacity simultaneously, which doubles the available
flow rate range.
Clicking Rename Ports makes it possible to rename the ports on the column
switching valves, buf
fer inlet valves, and sample inlet valves. For example, you can
r
ename the ports on the column switching valve to display the name of the columns
connected to each specific port. Once the ports are renamed, the new names
appear in the method in place of the valve port names.
Use Table 5 to select the default buffer mode for the method. The options available
depend on the fluidic scheme selected and the valves connected to your system. To
set the delay volume see System Settings on page 69.
Buffer Selection Modes
Buffer Inlet Type
Manually
Prepared
Manually Prepared via
Blending Valve
Buffer Blending
Pump Inlets

Pump + Inlet Valves

Pump + Buffer Blending
 
Pump + Buffer Blending + Inlet
Valves
  
If you are working with a fluidic scheme that has multiple inlet valves, you can
switch between buffers.
Note: Buffer blending requires a buffer blending valve.
Buffer blending can be used to dilute buffers. Define the pH of elution and select
buf
fers to achieve this pH.
Table 5. Buffer Selection Modes and Buffer Inlet Types