LIT162B 10/28/98 9:19 AM Page Cvr2 Protein Standard for Hydrophobic Chromatography Instruction Manual Catalog Number 151-1905 Bio-Rad Laboratories, 2000 Alfred Nobel Dr.
LIT162B 10/28/98 9:19 AM Page 1 For Calibration of Hydrophobic Columns The Protein Standard for Hydrophobic Chromatography is a lyophilized mixture of proteins which exhibit different hydrophobic behavior. It may be used as a calibration standard for HIC columns for analysis under nondenaturing conditions, and is appropriate for calibrating some reverse phase columns as well. The mixture contains cytochrome c, myoglobin and lysozyme.
LIT162B 10/28/98 9:20 AM Page 2 Protein Standard Components Conditions Column: Component Molecular Weight Cytochrome c 12,000 Myoglobin (horse) 17,000 Lysozyme 14,000 Recommended Volume of Standard Figure 1 shows separations of the standard using Hi-Pore® RP-304 HPLC columns. A volume of 20 µl standard was applied to each column. The protein peaks give absorbance at 280 nm from about 0.03 to 0.1 absorption units. Sample: Eluant: Flow Rate: Detection: Reversed Phase Hi-Pore RP-304, 250 x 4.
LIT162B 10/28/98 9:20 AM Page 4 Product Information Catalog Number Product Description 151-1905 Protein Standard for Hydrophobic Chromatography, 6 vials lyophilized mixture 125-0550 Hi-Pore RP-304 Reversed Phase HPLC Column, 250 x 4.
