Manual

2.9.2 Starter Kit
The Starter kit is included with each DuoFlow system. The kit includes step-by-step instructions for
programming and running a separation of a premixed anion exchange standard containing equine
myoglobin, conalbumin, chicken ovalbumin, and soybean trypsin inhibitor, using a 1.3 ml UNO Q1 Column.
The Starter kit includes the following items for running a separation:
• 50 ml of Buffer A, 250 mM Tris buffer pH 8.1 (10 X concentrate)
• 50 ml of Buffer B, 250 mM Tris buffer pH 8.1 plus 5.0 M NaCl (10 X concentrate)
• One vial of Anion Exchange Protein Standards (catalog number 125-0561)
• One 1-ml disposable sample injection syringe
• One 50 µl sample loop
• 50 ml of Maximizer solution A1 (10x concentrate)
• 50 ml of Maximizer solution A2 (10x concentrate)
• 50 ml of Maximizer solution B2 (2.5x concentrate)
The UNO Q1 Column (catalog number 720-0001) is not included with the Starter kit but is included with
each DuoFlow system.
For a complete discussion of the Starter kit, refer to its separate documentation.
2.9.3 Fittings kit, including Tubing kit
The DuoFlow Fittings kit is included with all DuoFlow system. It contains the following components:
• PEEK and PTFE Tubing
• Tefzel Plugs, Adaptors, Unions, and Caps
• Tubing Cutter
• 3 ml Syringe and 10 ml Luer Slip Syringe
• Molded Bottle Caps
• BioLogic Fittings Tool
• Screwdriver
• PEEK F to M and F to F Luers
• Delrin Nuts
• Super Flangeless Ferrules
• F10 Tubing Kit
Optional tubing kits include the following:
• F10 Tubing Kit
The F10 Tubing Kit includes precut 1/4-28 fitted PTFE, Tefzel, and 0.02” ID PEEK tubing for easy
installation of a DuoFlow basic systems. An installation chart indicates suggested placement of each
piece of labeled tubing (see Section 4.0).
The kit includes: a length of PTFE tubing, 1/8” OD, for connecting solution bottles to the Workstation
pumps A and B; a length of Tefzel tubing, 1/16” OD, for connecting AVR7-3 waste lines; and 8
additional pieces of PEEK, orange, 1/16” OD x 0.02” ID tubing, to connect the pump, mixer, column,
detector, and other components.
DESCRIPTION OF SYSTEM COMPONENTSSYSTEM OVERVIEW
2-47