Manual

• The BioFrac collects fractions in a serpentine pattern for all racks but may be changed to a row or
column pattern for microplates and microtiter tubes. Fourteen collection choices are possible with a
total of nine racks. The BioFrac accommodates 12-20 mm and 30 mm tube diameters, microtubes,
and scintillation vials in a variety of diameters. The optional ice bath/microplate rack allows collection
into 13 mm diameter tubes, microplates, and microtiter tubes that meet SBS standards required for
automated microplate systems. The drop head height can be adjusted to accomodate tubes up to
150 mm.
The following racks are available.
Table 2-12.
BioFrac Racks Available
Rack Name Description Capacity (Format)
F1 12-13 mm diameter tube; 2 racks (6x15), 180 tubes
150 mm height
F2 15-16 mm diameter tube; 2 racks (5x12), 120 tubes
150 mm height
F3 18-20 mm diameter tube; 2 racks (4x10), 80 tubes
150 mm height
H1 1.5-2.0 ml capless microtubes 4 racks (6x7), 168 tubes
H2 0.5 ml capless microtubes 4 racks (7x9), 120 tubes
H3 16 mm scintillation vials 4 racks (5x6), 120 tubes
H4 30 mm scintillation vials 4 racks (2x3), 24 vials
H4-High 30 mm tubes 4 racks (2x3), 24 tubes
Ice Bath, Ice bath for 13 mm diameter 1 rack (10x12), 120 tubes
Microplate rack tubes, standard microplate or 4 plates (12x8), 96 wells
microtiter tubes (SBS standard 4 plates (8x6), 48 wells
format) 4 plates (4x6), 24 wells
4 plates (3x4) 12 wells
Prep-20 Preparative rack bottle size fractions (20 funnels)
The BioFrac communicates with the DuoFlow Controller via the Instrument Bus. To connect the BioFrac to
the bitbus use either of the instrument bus connectors on the rear of the fraction collector. The BioFrac
fraction collector supports collection schemes such as Collect All, Threshold, Collection Windows, and
Threshold + Collection Windows (all with Delay volume, if required). The BioFrac diverter valve can be
mounted on either side of the fraction collector’s upright supports. See Section 3.8.1 for more information on
connecting the BioFrac to the DuoFlow system.
The BioFrac is plumbed to the DuoFlow system using 0.020” diameter PEEK tubing and 1/4-28 fittings for
flow rates up to 20 ml/min or with 0.030” diameter PEEK tubing and 1/4-28 fittings for flow rates up to
100 ml/min.
This instrument is described in detail in its separate documentation.
DESCRIPTION OF SYSTEM COMPONENTS SYSTEM OVERVIEW
2-36