Manual

Figure 7-5b. Run Screen showing a Run in Progress with a Time-based Chromatogram
and "Rack and Grid#" fraction numbering
Advance
Frac. Collector
Divert Valve
UV Detector
Set
UV Range
Zero
Baseline
Event
mark
Cond Range
500
Protocol: > 1 0:00.0 Collection Fractions of size 2.00 ml during entire run
Scout: Method 7 - Scout Run - [S] Scout Buffer A A1 [ 1 of 1 ] > [S] Scout Buffer A A1
Collect
Waste
Grad. Pump
Chart Recorder
1000
High psi
0
Low psi
QuadTec
Zero
Baseline
0.01
ConductivityUV
PauseAbort Hold
Full View
PULL DOWN MENU
TO SELECT LEFT TRACE
PULL DOWN MENU
TO SELECT RIGHT TRACE
ACTIVE
ACTIVE
BioLogic Duo-Flow - <user name> - <project name> - <method name> - <run name>
File View Utilities Options
Edit
Method
New
Method
New
Run Browser Manual Setup
Protocol
wash
load
1
2
Run
Notes
Report
PostRun
Log
Window Help
Settings
WL1 - 280nm
0.40
WL2 - 260nm
0.15
WL3 - 214nm
2.00
WL4 - 405nm
0.00
Flow Rate EGP %B
0 %B0.00 ml/min 0%
% Split
Run Time
0:00.0
Run Volume
0.0 ml
Step Time Left
0:00.0
Fraction Vol. Left
0.0 ml
WL1 - 280nm
Port 2: User Valve; Position 1
1.00ml/min 0 %B2
Maximizer + Gradient Pump: F10 UV Conductivity
438 psi 1.003 AU 1.23 mS/cm
QuadTec
Econo Gradient
Pump
SIM1/pHSIM1/SIG
0.548 Volt 7.00 pH
Edit
Bio-Rad
Web
100.0% Buffer B
50.0
0.00
1F
A
2F 3F 4F 5F 6F 7F 8F 9F 10F 11F 12F 13F14F
4.002.00 6.00
AU
mS/cm
0.0
50.0
40.0
30.0
20.0
10.0
-10.0
0.100
0.075
0.050
0.025
-0.000
Min. Tenth
Fractions
Rack Pos:
Grid #:
BA
MODES OF OPERATIONSYSTEM OPERATION
7-31