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Table 7-10. (continued)
Fraction Collection
Description: Threshold and Collection Windows
Threshold and Collection Windows: Each Collection Window (discussed on page 7-22) can also contain a
threshold value to further discriminate when eluant can be collected. For each collection window, non-peak
eluent can be directed to waste or to the fraction collector. The Threshold and Time windows can be
combined, so the system collects fractions only during the programmed Time windows and within the Time
windows only when the detector signal is above or below the threshold.
When using the Collection Windows functions, the BioFrac and Model 2128 will skip a tube between each
window collection. For example, if the first window ends collection at 20 minutes and the second window
starts collection starting at 21 minutes, then the fraction collector will leave an empty tube for the period
between minutes 20 and 21.
Collect All
Threshold
Collection Windows
Threshold and Collection Windows
Fraction Collection Scheme
Close
Waste
Destination:
Non-peak Parameters:
Tu be s
Below Threshold
Above Threshold
A
B90
Rack:
1
1A
1F
Tube #: Row Col.
Start:
End:
1.00
Fraction Size
ml
0.100
Threshold
1.00
4.00 5.00 0.60 1.00
Fraction Size
ml
UV Detector
Threshold Dectector
BioFrac Rack Type: F1 (12-13 mm tubes)
AU
Thresh.
(AU)
Non-Peak Frac. Size
(ml)
Start
(ml)
CollectionWindows
Fraction Collector Scheme
End
(ml)
Frac. Size
(ml)
1
2
7.00 8.00 1.00 0.000 Waste
Waste
1
2 7.00
Start
(ml)
Save Window
Finished Addin
g
8.00
CollectionWindowsFraction Collector Scheme
8.00
End
(ml)
1.00
1.00
4.00 5.00 0.60
Frac. Size
(ml)
1.00 0.200 0.01
Waste
Tu be s
0.0 4.0 1.00 1.00 0.01
Thresh.
(AU)
Thresh.
(AU)
Non-Peak Frac. Size
(ml)
Waste
Tu be s
Non-Peak Frac. Size
(ml)
Start
(ml)
Close
Save Changes
EDIT MODE
CollectionWindowsFraction Collector Scheme
End
(ml)
Frac. Size
(ml)
1
2
7.00 8.00 1.00 0.010 Waste
Delete Window
Cancel Editing
Close
Add Window
SELECT MODE
Delete Window
Close
ADD MODE
Delete Window
0.100
0.200 Waste
Waste
MODES OF OPERATIONSYSTEM OPERATION
7-23