Manual

Table 7-10.
Fraction Collection
Button Description: Collect All
To set up fraction collection. Fraction collection schemes are discussed below.
IMPORTANT NOTE:
The DuoFlow system is designed to control the BioFrac and the Model 2128 fraction collectors, which are
the only collectors that provide a choice of racks and the ability to overlay fractions from consecutive runs.
The DuoFlow system will also accommodate the Bio-Rad Model 2110 fraction collector as well as generic
collectors. To use a fraction collection scheme other than “Collect All” with these collectors, it is
essential to
assign a SVT3-2 valve as a “Fraction Collector Diverter” in the Setup screen. Rack choice and overlay
features are
not available with these collectors.
Collect All: The eluant from the entire run will be collected by the fraction collector.
• Fraction Size: Enter the fraction size as volume (ml).
• Tubes Required and Number of Racks: The displayed value is calculated based on the total volume
divided by fraction size.
• Start Tube and End Tube: Identifies the first and last tubes to receive fractions. This applies only to the
BioFrac and the Model 2128 fraction collector.
• Start Rack and End Rack: Identifies the first and last BioFrac rack to receive fractions.
Collect All
Threshold
Collection Windows
Threshold and Collection Windows
Fraction Collection Scheme
BioFrac Rack Type: F1 (12-13 mm tubes)
Close
Waste
Destination:
Non-peak Parameters:
Tubes
Below Threshold
Above Threshold
A
B 90
Rack:
1
1A
1F
Tube #: Row Col.
Start:
End:
1.00
Fraction Size
ml
0.100
Threshold
1.00
Fraction Size
ml
UV Detector
Threshold Detector
AU
MODES OF OPERATION SYSTEM OPERATION
7-20
Fraction
Collection