User Manual

Introduction

This instruction manual and starter kit contents may be used for the BioLogic

DuoFlow system and the BioLogic DuoFlow Maximizer

™

and Pathfinder

™

chromatography systems. The use of the starter kit with these systems is

described in Sections 1 and 3, respectively.

1. Starter Kit Components

This starter kit contains the following items for running a separation:

• 50 ml of buffer A, 250 mM Tris-HCI buffer, pH 8.1 (10x concentrate)

• 50 ml of buffer B, 250 mM Tris-HCI buffer, pH 8.1, plus 5.0 M NaCl (10x concentrate)

• 50 ml of Maximizer solution A1, 500 mM Tris-HCl (10x concentrate)

• 50 ml of Maximizer solution A2, 500 mM Tris base (10x concentrate)

• 50 ml of Maximizer solution B2, 5.0 M NaCl (2.5 x concentrate)

• One vial of anion exchange protein standard (catalog #125-0561)

• One 1 ml disposable sample injection syringe

• One 50 µl sample loop

The chromatographic separation for this kit requires approximately 6 minutes.

2. Materials You Will Need

In order to prepare the starter kit buffer solutions you will need the following

materials:

• Filtered high-quality water (i.e., HPLC grade water)

• One 500 ml graduated cylinder

• One 1 L side-arm flask

• Stirbar and stirplate

• Vacuum source for degassing

• Two 500 ml bottles

• Fraction collection tubes, 13 x 100 mm (at least 14 tubes)

• 100 ml beaker

4006208B.qxd 6/28/2004 11:03 AM Page 6