User Manual

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c. Equilibrate the column with 13 ml (10 column volumes) of pH 8.10 Tris (0% B).
When finished, the conductivity monitor on the status bar should read
≤ 3 mS/cm.
4.6 Create a New Method
In the Manual screen select the Browser icon from the tool bar. In the Browser
screen you will enter a user name and name for your method (refer to page 6-1
of the DuoFlow instruction manual for more information on the Browser screen)
according to the following steps:
• Select the Browser icon from the tool bar menu
• Select the New icon from the upper left side of the Browser screen
• Select New from the drop-down menu and enter your user name in the dialog
box
• Click on the Project icon for your user name
• Select New and New Method. Enter your method name (or use default
Method 1)
• Click OK to proceed to the instrument/devices Setup screen
Devices in setup
UV Detector
Buffer blender: Tris (25mM)
Signal Import Module 1 pH Range: 0.00 to 14.00 pH
BioFrac Fraction Collector, Rack: F1 (12-13 mm tubes)
SV5-4 Valve - Inlet A
SV5-4 Valve - Inlet B
Conductivity Monitor
AVR7-3 Valve - Sample Inject Port 10
Available Devices
Maximizer + Gradient Pump: F10
SVT3-2
Valve
AVR7-3
Valve
SV5-4
Valve
AVR9-8
Valve
Detectors
Fraction
Collector
SV3
INJECT
P
U
M
P
Aux Load
Pump
Inlet A1:
Inlet B1:
BioLogic Duo-Flow - <user name> - <project name> - <method name> - <run name>
Edit
Method
New
Method
New
Run Browser Manual Setup Delete
Protocol
wash
load
1
2
Run
Notes
Report
PostRun
Log
Settings
Port 1
Port 2
WL1 - 280nm
0.40 AU
WL2 - 260nm
0.15 AU
WL3 - 214nm
1.15 AU
WL4 - 405nm
0.30 AU
Flow Rate EGP %B
0 %B0.00 ml/min 0%
% Split
1.00ml/min
0 %B2
Gradient Pump: F10 UV Conductivity
438 psi 1.003 AU 1.23 mS/cm
QuadTec
Econo Gradient
Pump
SIM1/pHSIM1/SIG
0.548 Volt
7.00 pH
File View Utilities Options Window Help
Edit
Bio-Rad
Web
Buffer Blender
Is assigned to buffer blender
Is assigned to buffer blender
Inlet A2:
Inlet B2:
Is assigned to buffer blender
Is assigned to buffer blender
A1: 50 mM Tris-HCl; A2: 50 mM Tris
B1: Water; B2: 2.0M NaCl
Fig. 9. Setup editor
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