User Manual

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3.9 Status Bar
At the bottom of the Manual screen is a status bar that is continually updated
with system parameters.
Section 4. Anion Exchange Separation of Protein Standards
The starter kit enables you to learn to use the DuoFlow Maximizer or Pathfinder
systems by programming and running separation of a premixed anion exchange
standard containing equine myoglobin, conalbumin, chicken ovalbumin, and
soybean trypsin inhibitor, using a 1.3 ml UNO Q1 column (catalog #720-0001).
Equine myoglobin is not retained on the UNO Q1 column and elutes in the void
volume. Conalbumin, chicken ovalbumin, and soybean trypsin inhibitor bind to
the column and require increased salt concentrations for elution. Separation
requires approximately 6 minutes.
4.1 Overview of the Procedure
Run Conditions
• Buffer A1 50 mM Tris-HCl
• Buffer A2 50 mM Tris base
• Solution B1 deionized water
• Solution B2 2.0 M NaCI
• Flow rate 4.00 ml/min
• Sample volume 50 µl
• UV detection 0.1 AUFS
• QuadTec detection 0.1 AUFS (λ = 280 nm), 0.1 AUFS (λ = 260 nm),
1.0 AUFS (λ = 214nm), and 0.4 AUFS (λ = 304 nm)
• Conductivity 100 mS/cm
• pH pH 7.1 to pH 9.1
General Procedure
Step 1 Prepare solutions A
1
, A
2
, B
1
, and B
2
Step 2 Prepare sample
Step 3 Install the UNO Q1 column
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