Manual

Table 2. Macro-Prep High Q & S and
Macro-Prep DEAE specifications.
Properties High Q High S DEAE
Type of ion exchanger Strong anion Strong cation Weak anion
Functional group -N
+
(CH
3
)
3
-SO
3
-
-N
+
(C
2
H
5
)
2
Total ionic capacity 400 ± 75 µeq/ml 160 ± 40 µeq/ml 175 ± 75 µeq/ml
Dynamic binding capacity* ≥37 mg BSA/ml ≥49 mg IgG/ml ≥30 mg BSA/ml
Shipping counterion Cl
-
Na
+
Cl
-
Chemical stability
1% SDS, 24 hr Yes Yes Yes
6M guanidine-HCl, 24 hr Yes Yes Yes
pH stability 1–10 1–12 1–10
Total organic carbon in ppm, after 24 hours at 22°C
0.1 M HCl 0 4 0
0.2 M H
3
PO
4
0130
0.01 M NaOH 28 N/A N/A
0.1 M NaOH 200 31 26
1.0 M NaOH 337 49 121
Antimicrobial agent 20% ethanol 20% ethanol 20% ethanol
Regeneration 1–2 M NaCl 1–2 M NaCl 1–2 M NaCl
Storage conditions 20% ethanol 20% ethanol 20% ethanol
* 10% breakthrough capacity determined in a 1.1 x 20 cm column.
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