Manual

Table 4. Common buffers for ion exchange
chromatography.
Type of Buffering Buffer Range
Cation Exchanger
Acetic acid 4.8–5.2
Citric acid 4.2–5.2
HEPES 7.6–8.2
Lactic acid 3.6–4.3
MES 5.5–6.7
MOPS 6.5–7.9
Phosphate 6.7–7.6
PIPES 6.1–7.5
Pivalic acid 4.7–5.4
TES 7.2–7.8
Tricine 7.8–8.9
Anion Exchanger
Bicine 7.6–9.0
Bis-Tris 5.8–7.2
Diethanolamine 8.4–8.8
Diethylamine 9.5–11.5
L-Histidine 5.5–6.0
Imidazole 6.6–7.1
Pyridine 4.9–5.6
Tricine 7.8–8.9
Triethanolamine 7.3–8.0
Tris 7.5–8.0
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