Manual

Table 3. Products for buffer exchange.
Sample Recommended
Volume Product Use Catalog #
50–100 µl Bio-Spin 6 column Desalting proteins 732-6002
≥ 6 kD
50–100 µl Bio-Spin 30 column Desalting proteins 732-6006
≥ 30 kD
100 µl–3 ml Bio-Scale Mini P-6 Desalting proteins 732-4502
cartridge ≥ 6 kD
Up to 3 ml Econo-Pac 10DG Desalting proteins 732-2010
desalting column ≥ 6 kD
Unlimited Bio-Gel P-6DG gel Desalting proteins 150-0738
≥ 6 kD
4.2 General Purification Protocol
Ion exchange chromatography is usually performed
using increasing salt gradients or pH gradients to
elute the sample components. For best results and
to increase cartridge life, samples and buffers should
be degassed and filtered through a 0.45 µm filter.
Common buffers for cation and anion exchange
chromatography are listed in Table 4. An appropriate
starting point for purifying samples is a linear
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