User Manual

Table of Contents
Page
Section 1 Characteristics of the Bio-Scale CHT2, CHT5, CHT10, CHT20
Ceramic Hydroxyapatite, Type I Columns...............................................1
1.1 Introduction ................................................................................................................1
1.2 The CHT-I Separation Medium.................................................................................1
1.3 Connection to the BioLogic, FPLC
®
and HPLC Systems ........................................1
Section 2 Use of the Bio-Scale CHT-I Columns.........................................................2
2.1 Preparation for Initial Use..........................................................................................2
2.2 Sample Preparation ....................................................................................................3
2.3 Elution Conditions......................................................................................................3
2.4 Use of Detergents.......................................................................................................4
2.5 Effect of Calcium Chloride........................................................................................4
2.6 Chromatography Pre-Runs.........................................................................................4
Section 3 Care of the Bio-Scale CHT-I Columns ......................................................5
3.1 Column Hygiene ........................................................................................................5
3.2 Bed Height Adjustment..............................................................................................5
3.3 Frit Removal...............................................................................................................5
3.4 Top-Off Resin.............................................................................................................5
3.5 Storage Conditions.....................................................................................................5
3.6 References ..................................................................................................................8
Section 4 Product Information....................................................................................8