Software® Upgrades and Conversions Manual

Report Table
159
• Conc in Range — observed concentration that falls within the
range of standard concentrations, given a specified recovery
range. Values outside the range are flagged as ***. See page 160
for how Conc in Range relates to the Obs/Exp * 100 Column
• Obs Conc — observed concentration of the analyte, calculated
from its fluorescent intensity and the standard curve. Note that the
observed concentrations of standards are back-calculated from
their fluorescent intensities and the standard curve
• Obs Conc Std Dev — observed concentration of the analyte, with
the standard deviation of the replicate group of wells
• Obs Conc Std Err — observed concentration of the analyte, with
the standard deviation of a replicate group of wells, divided by the
square root of the number of replicates
• Obs Conc % CV — observed concentration of the analyte, with
coefficient of variation of a replicate group of wells. If this value is
high, you may want to review the individual FI values and select the
appropriate member of the replicate group as an outlier
• Exp Conc — expected concentration of the analyte standard or
control, as specified in the Enter Standards Info window or Enter
Controls Info window, respectively
• Obs/Exp * 100 — recovery rate for the observed versus expected
concentrations of the standard or control (see page 160)
• Group — defined group of the selected well (see page 72)
• Ratio — a calculation performed when you have grouped samples
in the plate formatting section. This calculation is the ratio of the
member-to-reference measured values, or vice versa. (For more
information, see Ratio (Simple) and the Grouping Function, on
page 226 of the Appendix)
• Dilution — specified dilution factor of the analyte
• Bead Count — number of beads counted in region for the analyte
• Bead Mean — mean fluorescent intensity of the beads counted in
region for the analyte
• Bead Std Dev — standard deviation of the bead mean
• Bead Std Err — standard error of the bead mean
• Bead %CV — coefficient of variation of the bead mean
• Trimmed Mean — bead mean, excluding the top 5% and bottom
5% of fluorescent intensities
• Trimmed Std Dev — standard deviation of the trimmed mean
• Trimmed Std Err — standard error of the trimmed mean
• Trimmed %CV — coefficient of variation of the trimmed mean
• Sampling Errors — error codes for a reading (see page 105)
BioPlex_6.book Page 159 Friday, September 23, 2011 11:55 AM