User Manual

1 x 96-Well 10 x 96-Well

Component Format Format

Standard diluent* 10 ml 100 ml

Sample diluent* 40 ml 80 ml

Assay buffer 50 ml 500 ml

Wash buffer 200 ml 1.5 L

Detection antibody diluent 5 ml 50 ml

Streptavidin-PE (100x) 1 tube 1 tube

Filter plate and/or ﬂat bottom plate (96-well) 1 plate 10 plates

Sealing tape 1 pack of 4 10 packs of 4

Assay Quick Guide 1 booklet 1 booklet

Standard 1 vial 10 vials

Human and Mouse Cytokine (Group I and II)

Coupled magnetic beads (10x) 1 tube 1 tube

Detection antibodies (10x) 1 tube 1 tube

Mouse Cytokine (Group III) and Rat Cytokine (Group I)

Coupled magnetic beads (20x) 1 tube 1 tube

Detection antibodies (20x) 1 tube 1 tube

* Bio-Plex Pro high dilution reagent kit, 1 x 96-well, contains 70 ml serum-based diluent in lieu of

standard diluent and sample diluent.

Kit Contents and Storage

Reagents Supplied

Bio-Plex Pro

™

cytokine assays are offered in a convenient kit format that

includes assay, reagent, and diluent components in a single box (Table 1).

Table 1. Contents of Bio-Plex Pro cytokine, chemokine, and growth factor assays.*

Storage and Stability

Kit contents should be stored at 4°C and never frozen. Coupled magnetic

beads and streptavidin-PE should be stored in the dark. All components

are guaranteed for a minimum of six months from the date of purchase

when stored as speciﬁed.